Same same but different: sperm-activating EC1 and ECA1 gametogenesis-related family proteins.
Same same but different: sperm-activating EC1 and ECA1 gametogenesis-related family proteins.
复制标题
DOI：
10.1042/bst20140039
复制
发表时间：
2014-04
影响因子：
3.9
通讯作者：
S. Sprunck;T. Hackenberg;Maria Englhart;F. Vogler
中科院分区：
文献类型：
--
作者：
S. Sprunck;T. Hackenberg;Maria Englhart;F. Vogler
在拟南芥的双受精过程中，卵细胞分泌富含半胱氨酸的EC1(卵细胞1)蛋白，这使得到达的精子对能够快速与两个雌配子相互作用。EC1蛋白是ECA1(早期培养丰富的1)配子发生相关蛋白家族中尚未被发现的成员，其特征是具有一个醇溶蛋白样域和六个保守的半胱氨酸残基，可形成三对二硫键。然而，卵细胞表达的EC1蛋白的区别标志是在所有类EC1蛋白中都存在两个短氨基酸序列基序。EC1基因似乎编码植物卵细胞表达的主要CRPS(富含半胱氨酸的蛋白)，它们仅限于开花植物，包括现存最基本的开花植物毛虫。许多其他ECA1配子发生相关家族基因在助细胞中优先表达。ECA1在雌配子体中的不同表达模式和低一级序列保守性表明，ECA1配子发生相关家族之间的功能多样化。
During double fertilization in Arabidopsis thaliana, the egg cell secretes small cysteine-rich EC1 (egg cell 1) proteins, which enable the arriving sperm pair to rapidly interact with the two female gametes. EC1 proteins are members of the large and unexplored group of ECA1 (early culture abundant 1) gametogenesis-related family proteins, characterized by a prolamin-like domain with six conserved cysteine residues that may form three pairs of disulfide bonds. The distinguishing marks of egg-cell-expressed EC1 proteins are, however, two short amino acid sequence motifs present in all EC1-like proteins. EC1 genes appear to encode the major CRPs (cysteine-rich proteins) expressed by the plant egg cell, and they are restricted to flowering plants, including the most basal extant flowering plant Amborella trichopoda. Many other ECA1 gametogenesis-related family genes are preferentially expressed in the synergid cell. Functional diversification among the ECA1 gametogenesis-related family is suggested by the different patterns of expression in the female gametophyte and the low primary sequence conservation.