Guidelines for interpreting EBER in situ hybridization and LMP1 immunohistochemical tests for detecting Epstein-Barr virus in Hodgkin lymphoma.
Guidelines for interpreting EBER in situ hybridization and LMP1 immunohistochemical tests for detecting Epstein-Barr virus in Hodgkin lymphoma.
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DOI：
10.1309/mmau-0qyh-7bha-w8c2
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发表时间：
2002-02
影响因子：
3.5
通讯作者：
M. Gulley;S. Glaser;F. Craig;M. Borowitz;R. Mann;S. Shema;R. Ambinder
中科院分区：
文献类型：
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作者：
M. Gulley;S. Glaser;F. Craig;M. Borowitz;R. Mann;S. Shema;R. Ambinder
组织化学染色显示约40%的霍奇金淋巴瘤中存在eb病毒（EBV），提示EBV在肿瘤发生中起作用，并具有EBV靶向治疗的潜力。随着研究的深入，确定组织化学染色的解释标准是很重要的。四名血液病理学家独立解释了40例霍奇金淋巴瘤的ebv编码RNA （EBER）和潜伏膜蛋白1 （LMP1）组织化学染色，然后将这些染色作为一组进行审查，以解决差异并制定解释指南。要将何杰金氏病例称为ebv相关，必须在RS/H细胞中明确存在EBER和/或LMP1信号。染色细胞的细胞学特征和分布应与相应的h&e染色载玻片相匹配，以帮助解释EBER或LMP1信号是恶性细胞还是反应性细胞。EBER信号定位于细胞核，而LMP1则定位于细胞质和表面膜。在某些情况下，由于技术或生物学原因，只有一小部分RS/H细胞表达这些因子。在称一个病例为eber阴性之前，必须证明肿瘤细胞RNA被保存下来并可用于杂交。LMP1染色，虽然通常在所有肿瘤细胞中都很强，但也可能是局部的和弱的，导致假阴性解释。对于ebv相关的霍奇金淋巴瘤，EBER和LMP1联合检测比单独检测更有效。
Histochemical stains demonstrate Epstein-Barr virus (EBV) in approximately 40% of all Hodgkin hymphomas, suggesting a role in tumorigenesis and the potentialfor EBV-targeted therapy. As research progresses, it is important to define criteria for interpreting histochemical stains. Four hematopathologists independently interpreted EBV-encoded RNA (EBER) and latent membrane protein 1 (LMP1) histochemical stains from 40 cases of Hodgkin lymphoma and then reviewed the stains as a group to resolve discrepancies and to develop interpretation guidelines. To call a Hodgkin case EBV-related, the EBER and/or LMP1 signal must be unequivocally present in Reed-Sternberg/Hodgkin (RS/H) cells. The cytologic features and distribution of stained cells should be matched with those on the corresponding H&E-stained slide to help interpret whether the EBER or LMP1 signal is in malignant or reactive cells. The EBER signal is localized to the nucleus, whereas LMP1 is in the cytoplasm and surface membrane. In some cases, only a fraction of RS/H cells express these factors for technical or biologic reasons. Before calling a case EBER-negative, it is essential to show that tumor cell RNA is preserved and available for hybridization. LMP1 staining, although usually strong among all tumor cells in a given case, may alternatively be focal and weak, contributing to false-negative interpretation. EBER and LMP1 assays in combination are more effective than either assay alone for identifying EBV-related Hodgkin lymphoma.