The 26-Killodalton, AhpC homologue, of Helicobacter pylori is also produced by other Helicobacter species
The 26-Killodalton, AhpC homologue, of Helicobacter pylori is also produced by other Helicobacter species
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DOI：
10.1046/j.1523-5378.2001.00006.x
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发表时间：
2001-03-01
期刊：
影响因子：
4.4
通讯作者：
Bölin, I
中科院分区：
文献类型：
--
作者：
Lundström, AM;Sundaeus, V;Bölin, I
背景26 kDa蛋白是烷基氢过氧化物还原酶（AhpC）同系物，早先已被描述为对幽门螺杆菌具有特异性。本研究的目的是分析是否这种蛋白质，或相应的基因，可以确定在其他螺杆菌物种。两种不同的单克隆抗体（Mab），其识别H. pylori中，用于免疫印迹以确定10种螺杆菌中蛋白质的存在。进行PCR以分析该基因是否可检测，并对PCR产物进行测序。为了比较幽门螺杆菌的基因和mRNA转录本，分别对从一些选择的螺杆菌物种中分离的染色体DNA和总RNA进行Southern和北方印迹分析。pylori.Results.在H. nemestrinae（primate），黑腹蛛H. acinonychis（cheetah），H. bills（mouse），H. felis（cat）和H. salomonis（dog），而H. mustelae（ferret）、白鼬H. cinaedi（human），H. canis（dog）、犬血吸虫H. fennelis（人）或H.鸡白痢（家禽）。通过PCR检测，该基因在H. mustelae，H. cinaedi和II.鸡白痢PCR产物与H.幽门。该基因在4个H. pylori菌株（94-99%同源性）。Southern杂交结果表明，黑腹蛛、黑腹蛛亚科和黑腹蛛属的黑腹蛛均为非寄主。Acinonychis染色体DNA含有该基因的单拷贝，北方印迹分析表明该基因在这两个物种中的单顺反子转录，与在H. pylori.Conclusions.在10种分析的螺杆菌中有8种发现了与ahpC相似的基因。
Background. The 26 kDa protein, which is an alkyl hydroperoxide reductase (AhpC) homologue, has earlier been described as specific for Helicobacter pylori. The aims of this study were to analyse whether this protein, or the corresponding gene, could be identified in other Helicobacter species.Materials and Methods. Two different monoclonal antibodies (Mabs), which recognise the 26 kDa protein in H. pylori, were used in immunoblots to determine the presence of the protein in 10 Helicobacter species. PCR was performed in order to analyse whether the gene was detectable and the PCR products were sequenced. Southern and Northern blot analyses were done on chromosomal DNA and total RNA, respectively, isolated from some selected Helicobacter species in order to compare the genes and mRNA transcripts to H. pylori.Results. The 26 kDa protein was identified in H. nemestrinae (primate), H. acinonychis (cheetah), H. bills (mouse), H. felis (cat) and H. salomonis (dog) but not in H. mustelae (ferret), H. cinaedi (human), H. canis (dog), H. fennelliae (human) or H. pullorum (poultry). By PCR the gene was also recognised in H. mustelae, H. cinaedi and II. pullorum. The PCR products showed high sequence homology (66-98%) compared to H. pylori. The gene was also highly conserved in four H. pylori strains (94-99% homology). Southern blot showed that the H, nemestrinae and H. acinonychis chromosomal DNA contained a single copy of the gene and the Northern blot analyses indicated mono-cistronic transcription of the gene in these two species, as has been found in H. pylori.Conclusions. A gene similar to ahpC was found in eight out of 10 Helicobacter species analysed.