Strong Correlation Between Concentrations of Tenofovir (TFV) Emtricitabine (FTC) in Hair and TFV Diphosphate and FTC Triphosphate in Dried Blood Spots in the iPrEx Open Label Extension: Implications for Pre-exposure Prophylaxis Adherence Monitoring
Strong Correlation Between Concentrations of Tenofovir (TFV) Emtricitabine (FTC) in Hair and TFV Diphosphate and FTC Triphosphate in Dried Blood Spots in the iPrEx Open Label Extension: Implications for Pre-exposure Prophylaxis Adherence Monitoring
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DOI：
10.1093/infdis/jiv239
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发表时间：
2015-11-01
影响因子：
6.4
通讯作者：
Grant, Robert M.
中科院分区：
文献类型：
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作者：
Gandhi, Monica;Glidden, David V.;Grant, Robert M.
自我报告的暴露前预防（PrEP）依从性有局限性，引起了对药理学监测的兴趣。毛发和干血斑（DBS）中的药物浓度用于评估长期暴露;毛发运输/储存在室温下进行。iPrEx开放标签扩展定期采集DBS，选择性采集毛发;采用液相色谱/串联质谱法测量浓度。在806对头发-DBS中，替诺福韦（TFV）头发水平和DBS中的TFV二磷酸盐（DP）强烈相关（斯皮尔曼系数r = 0.734; P < .001），头发TFV/DBS恩曲他滨（FTC）三磷酸盐（TP）（r = 0.781; P < .001）;毛发FTC/DBS TFV-DP（r = 0.74; P < .001）;毛发FTC/DBS FTC-TP（r = 0.587; P < .001）。基质的药物可检测性通常一致。头发TFV/FTC浓度与DBS水平密切相关，DBS水平可预测PrEP结局。
Self-reported adherence to pre-exposure prophylaxis (PrEP) has limitations, raising interest in pharmacologic monitoring. Drug concentrations in hair and dried blood spots (DBS) are used to assess long-term-exposure; hair shipment/storage occurs at room temperature. The iPrEx Open Label Extension collected DBS routinely, with opt-in hair collection; concentrations were measured with liquid chromatography/tandem mass spectrometry. In 806 hair-DBS pairs, tenofovir (TFV) hair levels and TFV diphosphate (DP) in DBS were strongly correlated (Spearman coefficient r = 0.734; P < .001), as were hair TFV/DBS emtricitabine (FTC) triphosphate (TP) (r = 0.781; P < .001); hair FTC/DBS TFV-DP (r = 0.74; P < .001); hair FTC/DBS FTC-TP (r = 0.587; P < .001). Drug detectability was generally concordant by matrix. Hair TFV/FTC concentrations correlate strongly with DBS levels, which are predictive of PrEP outcomes.