Three restriction points in the cell cycle of activated murine B lymphocytes.
Three restriction points in the cell cycle of activated murine B lymphocytes.
复制标题
活化的鼠 B 淋巴细胞细胞周期中的三个限制点。
DOI：
10.1073/pnas.82.22.7681
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发表时间：
1985
影响因子：
11.1
通讯作者：
Lernhardt,W
中科院分区：
文献类型：
--
作者：
Melchers,F;Lernhardt,W
激活的B淋巴细胞的细胞周期受三个限制点的控制。第一种是在有丝分裂后立即发生，并被发现由琼脂糖结合的免疫球蛋白特异性抗体与表面膜结合的免疫球蛋白结合控制。在短至15分钟或长至36小时的刺激下，B细胞可以进入G1期，直到下一个限制点。第二个限制点约等于有丝分裂后4小时、细胞周期的G1期和B细胞进入S期前3-4小时，受P388D_1巨噬细胞产生的α型B细胞生长因子控制。第三个限制点出现在G2期，即有丝分裂前2-4小时，显然是由辅助T淋巴细胞产生的β-B细胞生长因子控制的。
The cell cycle of activated B lymphocytes was found to be controlled by three restriction points. The first occurs immediately after mitosis and was found to be controlled by the binding of Sepharose-bound, immunoglobulin-specific antibodies to surface membrane-bound immunoglobulin. Exposure to this stimulus as short as 15 min or as long as 36 hr allowed B cells to move into the G1 phase up to the next restriction point. The second restriction point was observed to be approximately equal to 4 hr after mitosis, in the G1 phase of the cycle and 3-4 hr before the B cells entered S phase, and was found to be controlled by alpha-type B-cell growth factors produced by the P388D1 macrophage line. A third restriction point occurs in the G2 phase, 2-4 hr before mitosis, and is apparently controlled by beta-type B-cell growth factors that are likely to be produced by helper T lymphocytes.