Association between friction and wear in diarthrodial joints lacking lubricin.
Association between friction and wear in diarthrodial joints lacking lubricin.
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DOI：
10.1002/art.22974
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发表时间：
2007-11
影响因子：
--
通讯作者：
Warman, Matthew L
中科院分区：
文献类型：
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作者：
Jay, Gregory D;Torres, Jahn R;Rhee, David K;Helminen, Heikki J;Hytinnen, Mika M;Cha, Chung-Ja;Elsaid, Khaled;Kim, Kyung-Suk;Cui, Yajun;Warman, Matthew L
糖蛋白润滑素（由基因Prg 4编码）由表面软骨细胞和滑膜细胞分泌，并已显示出在体外减少摩擦。与人造轴承相反，哺乳动物的关节必须内源性地产生减摩剂。本研究旨在调查摩擦是否与磨损相关。在体外测试了来自具有遗传性润滑素缺乏症的人的滑液（SF）样品的润滑能力。离体测量正常小鼠和无润滑素小鼠膝关节的摩擦系数;还通过光学显微镜和电子显微镜研究了这些关节。原子力显微镜用于成像和测量润滑素如何在体外减少摩擦。缺乏润滑的SF不能降低边界模式中的摩擦。与野生型小鼠相比，无润滑素小鼠的关节显示出早期磨损和更高的摩擦力。Lubricin自组织并减少了对置微凸体之间的粘附功。这些数据表明，在体内的软骨表面的摩擦与磨损耦合。这意味着在炎症性关节疾病中发生的获得性润滑素降解使软骨易于受损。最后，他们建议润滑素或类似的生物分子将在人造设备中应用，其中减少摩擦是必不可少的。
The glycoprotein lubricin (encoded by the gene Prg4) is secreted by surface chondrocytes and synovial cells, and has been shown to reduce friction in vitro. In contrast to man-made bearings, mammalian diarthrodial joints must endogenously produce friction-reducing agents. This study was undertaken to investigate whether friction is associated with wear. The lubricating ability of synovial fluid (SF) samples from humans with genetic lubricin deficiency was tested in vitro. The coefficient of friction in the knee joints of normal and lubricin-null mice was measured ex vivo; these joints were also studied by light and electron microscopy. Atomic force microscopy was used to image and measure how lubricin reduces friction in vitro. SF lacking lubricin failed to reduce friction in the boundary mode. Joints of lubricin-null mice showed early wear and higher friction than joints from their wild-type counterparts. Lubricin self-organized and reduced the work of adhesion between apposing asperities. These data show that friction is coupled with wear at the cartilage surface in vivo. They imply that acquired lubricin degradation occurring in inflammatory joint diseases predisposes the cartilage to damage. Lastly, they suggest that lubricin, or similar biomolecules, will have applications in man-made devices in which reducing friction is essential.