Nrf2 Deficiency Promotes Melanoma Growth and Lung Metastasis.
Nrf2 Deficiency Promotes Melanoma Growth and Lung Metastasis.
复制标题
DOI：
10.20455/ros.2016.853
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Li YR
中科院分区：
文献类型：
--
作者：
Zhu H;Jia Z;Trush MA;Li YR
抗氧化和细胞保护基因的关键调节因子Nrf 2在肿瘤发生中的作用仍存在争议。在这里，我们发现Nrf 2缺陷导致皮下注射B16-F10黑色素瘤细胞后小鼠局部肿瘤生长增加，如具有局部可触及肿瘤质量的动物比例增加和注射部位肿瘤体积的时间依赖性增加所示。体内生物发光成像也显示与野生型小鼠相比，Nrf 2基因敲除小鼠中黑色素瘤的生长增加。通过使用高灵敏度的生物发光测定，我们进一步发现，与野生型小鼠相比，Nrf 2缺陷导致B16-F10黑色素瘤细胞肺转移显著增加。总之，这一简短交流的结果首次证明，Nrf 2缺陷促进了小鼠皮下接种B16-F10细胞后黑色素瘤的生长和肺转移。
The role of Nrf2, a key regulator of antioxidant and cytoprotective genes, in tumorigenesis remains controversial. Here we showed that Nrf2 deficiency led to increased local tumor growth in mice following subcutaneous injection of B16-F10 melanoma cells, as indicated by increased proportion of animals with locally palpable tumor mass and time-dependent increases in tumor volume at the injection site. In vivo bioluminescence imaging also revealed increased growth of melanoma in Nrf2-null mice as compared with wild-type mice. By using a highly sensitive bioluminometric assay, we further found that Nrf2 deficiency resulted in a remarkable increase in lung metastasis of B16-F10 melanoma cells as compared with wild-type mice. Taken together, the results of this short communication for the first time demonstrated that Nrf2 deficiency promoted melanoma growth and lung metastasis following subcutaneous inoculation of B16-F10 cells in mice.