Molecular characterization and expression analysis of five different elongation factor 1 alpha genes in the flatfish Senegalese sole (Solea senegalensis Kaup):: Differential gene expression and thyroid hormones dependence during metamorphosis
Molecular characterization and expression analysis of five different elongation factor 1 alpha genes in the flatfish Senegalese sole (Solea senegalensis Kaup):: Differential gene expression and thyroid hormones dependence during metamorphosis
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DOI：
10.1186/1471-2199-9-19
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发表时间：
2008-01-30
影响因子：
--
通讯作者：
Manchado, Manuel
中科院分区：
文献类型：
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作者：
Infante, Carlos;Asensio, Esther;Manchado, Manuel
背景：真核细胞延伸因子1 α（eEF 1A）是真核细胞翻译延伸因子1的四个亚基之一。在蛋白质合成过程中，它以GTP依赖的方式催化氨酰-tRNA与核糖体A位点的结合，尽管它似乎也在其他非翻译过程中发挥作用。目前，关于其在比目鱼变态过程中的表达谱和调控的信息仍然很少。关于这一点，塞内加尔唯一的（Solea senegalensis）是一个商业上重要的比目鱼，其中eEF 1A基因仍然是characteristic.Results：塞内加尔唯一的大规模基因组学的发展促进了五个不同的eEF 1A基因的鉴定，被称为SseEF 1A 1，SseEF 1A 2，SseEF 1A 3，SseEF 1A 4，和Sse 42 Sp 50。主要特征和与其他鱼类和哺乳动物eEF 1A的序列同一性进行了描述。系统发育和组织表达分析允许鉴定SseEF 1A 1和SseEF 1A 2作为塞内加尔唯一对应的哺乳动物eEF 1A 1和eEF 1A 2，分别和Sse 42 Sp 50作为非洲爪蟾和硬骨鱼42 Sp 50基因的直系同源物。另外两个延伸因子SseEF 1A 3和SseEF 1A 4代表主要在鳃和皮肤中表达的新基因。这五个基因的表达谱也在幼虫发育过程中进行了研究，揭示了不同的行为。为了研究甲状腺激素（TH）对SseEF 1A基因表达的可能调控，将幼虫暴露于致甲状腺肿硫脲（TU）。TU处理的幼虫表现出较低的SseEF 1A 4 mRNA水平比未处理的对照组在11和15天后处理，而其他四个基因的转录保持相对不变。此外，除了外源性T4激素TU处理的幼虫的SseEF 1A 4的稳态水平显着增加相对于未处理的控制，表明其表达上调THs.Conclusion：我们已经确定了五个不同的eEF 1A基因在塞内加尔唯一的，被称为SseEF 1A 1，SseEF 1A 2，SseEF 1A 3，SseEF 1A 4，和Sse 42 Sp 50。这五个基因在组织和幼虫发育过程中表现出不同的表达模式。TU和T4处理表明，SseEF 1A 4是上调TH，这表明在翻译调节的因素参与的戏剧性的变化，发生在塞内加尔唯一的变态的作用。
Background: Eukaryotic elongation factor 1 alpha (eEF1A) is one of the four subunits composing eukaryotic translation elongation factor 1. It catalyzes the binding of aminoacyl-tRNA to the A-site of the ribosome in a GTP-dependent manner during protein synthesis, although it also seems to play a role in other non-translational processes. Currently, little information is still available about its expression profile and regulation during flatfish metamorphosis. With regard to this, Senegalese sole (Solea senegalensis) is a commercially important flatfish in which eEF1A gene remains to be characterized.Results: The development of large-scale genomics of Senegalese sole has facilitated the identification of five different eEF1A genes, referred to as SseEF1A1, SseEF1A2, SseEF1A3, SseEF1A4, and Sse42Sp50. Main characteristics and sequence identities with other fish and mammalian eEF1As are described. Phylogenetic and tissue expression analyses allowed for the identification of SseEF1A1 and SseEF1A2 as the Senegalese sole counterparts of mammalian eEF1A1 and eEF1A2, respectively, and of Sse42Sp50 as the ortholog of Xenopus laevis and teleost 42Sp50 gene. The other two elongation factors, SseEF1A3 and SseEF1A4, represent novel genes that are mainly expressed in gills and skin. The expression profile of the five genes was also studied during larval development, revealing different behaviours. To study the possible regulation of SseEF1A gene expressions by thyroid hormones (THs), larvae were exposed to the goitrogen thiourea (TU). TU-treated larvae exhibited lower SseEF1A4 mRNA levels than untreated controls at both 11 and 15 days after treatment, whereas transcripts of the other four genes remained relatively unchanged. Moreover, addition of exogenous T4 hormone to TU-treated larvae increased significantly the steady-state levels of SseEF1A4 with respect to untreated controls, demonstrating that its expression is upregulated by THs.Conclusion: We have identified five different eEF1A genes in the Senegalese sole, referred to as SseEF1A1, SseEF1A2, SseEF1A3, SseEF1A4, and Sse42Sp50. The five genes exhibit different expression patterns in tissues and during larval development. TU and T4 treatments demonstrate that SseEF1A4 is up-regulated by THs, suggesting a role in the translational regulation of the factors involved in the dramatic changes that occurs during Senegalese sole metamorphosis.