Human β-defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung
Human β-defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung
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DOI：
10.1172/jci2410
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发表时间：
1998-09-01
影响因子：
15.9
通讯作者：
Wilson, JM
中科院分区：
文献类型：
--
作者：
Bals, R;Wang, XR;Wilson, JM
以前的研究表明新型肽类抗生素人β-防御素1（hBD-1）与囊性纤维化的发病机制有关。本文报道了该防御素家族的第二个成员人β-防御素2（hBD-2）的分离和鉴定。通过与hBD-1的同源性鉴定hBD-2的cDNA。hBD-2在包括肺在内的许多器官的上皮细胞中弥漫性表达，在肺中发现hBD-2存在于粘膜下腺体的表面上皮细胞和浆液细胞中。用重组肽制备的特异性抗体检测人肺气道表面液中hBD-2。完全加工的肽对许多生物体具有广泛的抗菌活性，其是盐敏感的并且与溶菌酶和乳铁蛋白协同。这些数据表明在粘膜表面上存在β-防御素分子家族，其聚集在一起有助于正常的宿主防御。
Previous studies have implicated the novel peptide antibiotic human beta-defensin 1 (hBD-1) in the pathogenesis of cystic fibrosis, We describe in this report the isolation and characterization of the second member of this defensin family, human beta-defensin 2 (hBD-2). A cDNA for hBD-2 was identified by homology to hBD-1. hBD-2 is expressed diffusely throughout epithelia of many organs, including the lung, where it is found in the surface epithelia and serous cells of the submucosal glands. A specific antibody made of recombinant peptide detected hBD-2 in airway surface fluid of human lung. The fully processed peptide has broad antibacterial activity against many organisms, which is salt sensitive and synergistic with lysozyme and lactoferrin, These data suggest the existence of a family of beta-defensin molecules on mucosal surfaces that in the aggregate contributes to normal host defense.