Mitochondrial protein import pathways are functionally conserved among eukaryotes despite compositional diversity of the import machineries
Mitochondrial protein import pathways are functionally conserved among eukaryotes despite compositional diversity of the import machineries
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DOI：
10.1515/hsz-2011-0255
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发表时间：
2012-05-01
影响因子：
3.7
通讯作者：
Deponte, Marcel
中科院分区：
文献类型：
--
作者：
Eckers, Elisabeth;Cyrklaff, Marek;Deponte, Marcel
线粒体蛋白质输入（MPI）是真核生物线粒体生物合成的关键。目前的MPI模型主要是基于一组真核生物的实验，后突生物。虽然迷人的基因组数据库驱动的MPI机器的进化假说已经出版，以前的实验研究非opisthokonts通常集中在分析单一的途径或组件，例如，植物和寄生虫。在这项研究中，我们已经建立了动质体寄生虫利什曼原虫tarentolae作为一个模式生物的全面分析的非后鞭毛MPI到所有四个线粒体舱室。我们发现后鞭毛体标记蛋白被有效地导入到分离的L。狼蛛线粒体反之亦然，L。所有区室的tarentolae标记蛋白也从酵母输入线粒体。结果是显着的，因为只有少数的超过25个经典组件的后鞭毛MPI机器中发现的寄生虫基因组数据库。我们的研究结果表明，不同的MPI途径是功能保守的真核生物，尽管显着的组成差异的MPI机器。此外，我们的模型系统可以导致显着改变，甚至新的MPI组件在非opisthokonts的识别。这种差异可能作为开发抗寄生原生生物药物的起点。
Mitochondrial protein import (MPI) is essential for the biogenesis of mitochondria in all eukaryotes. Current models of MPI are predominantly based on experiments with one group of eukaryotes, the opisthokonts. Although fascinating genome database-driven hypotheses on the evolution of the MPI machineries have been published, previous experimental research on non-opisthokonts usually focused on the analysis of single pathways or components in, for example, plants and parasites. In this study, we have established the kinetoplastid parasite Leishmania tarentolae as a model organism for the comprehensive analysis of non-opisthokont MPI into all four mitochondrial compartments. We found that opisthokont marker proteins are efficiently imported into isolated L. tarentolae mitochondria. Vice versa, L. tarentolae marker proteins of all compartments are also imported into mitochondria from yeast. The results are remarkable because only a few of the more than 25 classical components of the opisthokont MPI machineries are found in parasite genome databases. Our results demonstrate that different MPI pathways are functionally conserved among eukaryotes despite significant compositional differences of the MPI machineries. Moreover, our model system could lead to the identification of significantly altered or even novel MPI components in non-opisthokonts. Such differences might serve as starting points for drug development against parasitic protists.