Amyloid-β-induced chemokine production in primary human macrophages and astrocytes
Amyloid-β-induced chemokine production in primary human macrophages and astrocytes
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DOI：
10.1016/s0165-5728(02)00112-1
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发表时间：
2002-06-01
影响因子：
3.3
通讯作者：
Nottet, HSLM
中科院分区：
文献类型：
--
作者：
Smits, HA;Rijsmus, A;Nottet, HSLM
In Alzheimer's disease (AD), chemotaxis might be responsible for attracting glial cells towards the neuritic plaque. Using primary monocyte-derived macrophages and primary adult astrocytes as a model, amyloid-beta (Abeta) (1-42) was able to stimulate the production, as measured by RT-PCR, of MIP-1alpha and MIP-1beta mRNA in macrophages and MCP-1 in astrocytes. Cocultures showed in unstimulated as well as in A beta-stimulated cells an increase in MIP-1alpha, MIP-1beta and MCP-1 mRNA. ELISAs of supernatant samples of stimulated macrophages and astrocytes also showed an increase in MIP-1alpha and MIP-1beta in macrophages and MCP-1 in astrocytes. Stimulated cocultures showed an increase in MIP-1alpha, MIP-1beta and MCP-1 protein levels in contrast to unstimulated cocultures. (C) 2002 Elsevier Science B.V. All rights reserved.
