Enhanced degradation of stimulatory G-protein (Gs alpha) by cholera toxin is mediated by ADP-ribosylation of Gs alpha protein but not by increased cyclic AMP levels.
Enhanced degradation of stimulatory G-protein (Gs alpha) by cholera toxin is mediated by ADP-ribosylation of Gs alpha protein but not by increased cyclic AMP levels.
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霍乱毒素对刺激性 G 蛋白 (Gs α) 的增强降解是由 Gs α 蛋白的 ADP 核糖基化介导的，而不是由环 AMP 水平增加介导的。
DOI：
10.1007/978-1-4419-8632-0_11
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发表时间：
1997
影响因子：
--
通讯作者：
B. H. Shah
中科院分区：
文献类型：
--
作者：
B. H. Shah
Cholera toxin (CT) catalyses ADP-ribosylation of the alpha-subunit of stimulatory protein (Gs) leading to stimulation of adenylyl cyclase and elevated intracellular cAMP. Persistent treatment (24-48 h) of C6 glioma cells with cholera toxin (100 ng/ml) caused marked downregulation of Gs alpha (75-80%) which could not be mimicked by dibutyryl cAMP (1 mM) and forskolin (10 microM) over the same time periods suggesting that CT-mediated Gs alpha downregulation is independent of cAMP production. However, CT increased the expression of Gq/11 alpha proteins at 24 and 48 h of treatment. The increase in mRNA levels of Gq/11 alpha proteins preceded the increase in Gq/11 proteins. Such stimulatory effects of CT were mimicked by forskolin and dibutyryl-cAMP. These results suggest that CT-mediated downregulation of Gs alpha is independent of cAMP but CT upregulates the expression of Gq/11 alpha proteins in a cAMP-dependent manner.
霍乱毒素诱导 Gs 的不依赖于 cAMP 的降解。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chang,FH;Bourne,HR
通讯作者：
Bourne,HR
DOI：
10.1016/s0021-9258(18)77181-0
发表时间：
1990-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Hadcock;M. Ros;D. C. Watkins;C. Malbon
通讯作者：
J. Hadcock;M. Ros;D. C. Watkins;C. Malbon