EXCITATORY AMINO-ACID PROJECTIONS TO THE PERIAQUEDUCTAL GRAY IN THE RAT - A RETROGRADE TRANSPORT STUDY UTILIZING D[H-3]ASPARTATE AND [H-3] GABA
EXCITATORY AMINO-ACID PROJECTIONS TO THE PERIAQUEDUCTAL GRAY IN THE RAT - A RETROGRADE TRANSPORT STUDY UTILIZING D[H-3]ASPARTATE AND [H-3] GABA
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DOI：
10.1016/0306-4522(90)90310-z
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发表时间：
1990-01-01
期刊：
影响因子：
3.3
通讯作者：
CHRISTIE, MJ
中科院分区：
文献类型：
--
作者：
BEART, PM;SUMMERS, RJ;CHRISTIE, MJ
已通过微量输注和逆行转运 d[3H]天冬氨酸后的放射自显影术描述了大鼠脑中利用兴奋性氨基酸递质向导水管周围灰质的传入。使用注射[3H]GABA的平行实验证实，用d[3H]天冬氨酸发现的逆行标记是递质选择性的。注射d[3H]天冬氨酸后，在九个皮层下区域发现核周标记，特别是边缘下皮层和扣带皮层，主要是同侧标记的核周。 Heaviest cortical labelling was localized in perirhinal cortex, in an extensive band of cells adjoining the rhinal sulcus.下丘脑含有脑内最重的核周标记：11 个下丘脑和乳头核中的d[3H]天冬氨酸标记细胞。尽管对侧核周的数量较低，但在下丘脑腹内侧获得了强烈的双侧标记。d[3H]天冬氨酸也在下丘脑后部产生同侧核周的大量标记。 Labelling patterns in cortex and hypothalamus were precise and topographic, and [3H]GABA never labelled cells in these regions.其他含有突出的逆行标记细胞的端脑和间脑区域是外侧隔膜、杏仁核、未定带和外侧缰核。尽管[3H]天冬氨酸标记了下丘和楔状核中中等数量的核周细胞，但中脑区域中标记细胞的相对密度远低于皮质和下丘脑中的相对密度。 A smaller number of heavily labelled cells was found in the parabrachial nuclei, Kolliker-Fuse nucleus and laterodorsal tegmental nucleus. Only occasional labelled perikarya were observed in the myencephalon.将[3H]GABA注射到导水管周围灰质后，发现标记细胞密度较低，并且少数核周细胞同时被[3H]GABA和d[3H]天冬氨酸标记的区域是中缝背核和臂旁核。总体而言，d[3H]天冬氨酸的逆行转运揭示了到导水管周围灰质的传入通路的复杂地形和会聚网络，可能利用兴奋性氨基酸递质。我们的研究结果证实了这种神经化学图谱技术的选择性，并提供了下丘脑、缰核、下丘脑和楔形传入导水管周围灰质利用酸性氨基酸作为其递质的证据。 They also confirm that corticofugal afferents to periaqueductal gray utilize an excitatory amino acid.
The afferents to the periaqueductal gray utilizing excitatory amino acid transmitters have been described in rat brain by autoradiography following microinfusion and retrograde transport ofd[3H]aspartate. Parallel experiments employing injections of [3H]GABA established that the retrograde labelling found withd[3H]aspartate was transmitter-selective.Following infusion ofd[3H]aspartate, perikaryal labelling was found in nine subcortical areas, particularly infralimbic and cingulate cortices, with a predominance of ipsilateral labelled perikarya. Heaviest cortical labelling was localized in perirhinal cortex, in an extensive band of cells adjoining the rhinal sulcus. The hypothalamus contained the heaviest perikaryal labelling within brain:d[3H]aspartate labelled cells in 11 hypothalamic and mammillary nuclei. Intense bilateral labelling was obtained in ventromedial hypothalamus, although the number of perikarya was lower contralaterally.d[3H]Aspartate also produced heavy ipsilateral labelling of perikarya in posterior hypothalamus. Labelling patterns in cortex and hypothalamus were precise and topographic, and [3H]GABA never labelled cells in these regions. Other telencephalic and diencephalic areas containing prominent, retrogradely labelled cells were the lateral septum, amygdala, zona incerta and lateral habenula. The relative density of labelled cells in mesencephalic areas was much lower than that found in cortex and hypothalamus, althoughd[3H]aspartate labelled a moderate number of perikarya in the inferior colliculus and cuneiform nucleus. A smaller number of heavily labelled cells was found in the parabrachial nuclei, Kolliker-Fuse nucleus and laterodorsal tegmental nucleus. Only occasional labelled perikarya were observed in the myencephalon. Low densities of labelled cells were found after the injection of [3H]GABA into the periaqueductal gray, and the only regions in which a small number of perikarya were labelled by both [3H]GABA andd[3H]aspartate were the dorsal raphe and parabrachial nuclei. Overall, the retrograde transport ofd[3H]aspartate revealed a complex topographic and convergent network of afferent pathways to the periaqueductal gray likely to utilize an excitatory amino acid transmitter.Our findings confirm the selectivity of this neurochemical mapping technique and provide evidence that hypothalamic, habenular, subthalamic and cuneiform afferents to the periaqueductal gray utilize an acidic amino acid as their transmitter. They also confirm that corticofugal afferents to periaqueductal gray utilize an excitatory amino acid.