CDNA CLONING AND EXPRESSION OF CRY-J-II, THE 2ND MAJOR ALLERGEN OF JAPANESE CEDAR POLLEN
CDNA CLONING AND EXPRESSION OF CRY-J-II, THE 2ND MAJOR ALLERGEN OF JAPANESE CEDAR POLLEN
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DOI：
10.1006/bbrc.1994.1804
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发表时间：
1994-06-15
影响因子：
3.1
通讯作者：
KINO, K
中科院分区：
文献类型：
--
作者：
KOMIYAMA, N;SONE, T;KINO, K
我们已经分离和测序的cDNA克隆编码的哭J II，日本雪松（柳杉）花粉的第二个主要过敏原。1.7-腺苷酸酶cDNA克隆含有一个开放阅读框架，编码514个氨基酸的蛋白质，包括一个54个氨基酸的推定信号肽和三个潜在的糖基化位点。Cry j II的推导的氨基酸序列与番茄（40%）和鳄梨（43%）中与果实成熟相关的多聚半乳糖醛酸酶以及玉米花粉中发现的多聚半乳糖醛酸酶（34%）的氨基酸序列具有显著的同一性。Cry j Ⅱ cDNA产物在E.结果表明，重组Cry j Ⅱ可被雪松花粉症患者的IgE所识别，提示重组Cry j Ⅱ可作为雪松花粉症的过敏原用于临床诊断。(C)1994年出版社出版。
We have isolated and sequenced a cDNA clone coding for Cry j II, the second major allergen of Japanese cedar (Cryptomeria japonica) pollen. A 1.7-kilobase cDNA clone contained an open reading frame coding for a 514-amino acid protein, including a putative signal peptide of 54 amino acid and three potential glycosylation sites. The deduced amino acid sequence of the Cry j II shows significant identities to those of the polygalacturonases associated with fruit-ripening in tomato (40%) and avocado (43%) and found in pollen of maize (34%). Cry j II cDNA product expressed in E. coli was recognized by human IgE from patients suffering from cedar pollinosis, suggesting that recombinant Cry j II might be used as an allergen for the clinical diagnosis of cedar pollinosis. (C) 1994 Academic Press, Inc.