Effects of combinations of transforming growth factor-beta 1 and tumor necrosis factor on induction of differentiation of human myelogenous leukemic cell lines.
Effects of combinations of transforming growth factor-beta 1 and tumor necrosis factor on induction of differentiation of human myelogenous leukemic cell lines.
复制标题
转化生长因子-β1与肿瘤坏死因子组合对人骨髓性白血病细胞系诱导分化的影响。
DOI：
10.4049/jimmunol.144.4.1311
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发表时间：
1990
影响因子：
4.4
通讯作者：
K. Konno
中科院分区：
文献类型：
--
作者：
R. Kamijo;K. Takeda;M. Nagumo;K. Konno
研究了转化生长因子-β 1（TGF-β 1）单独或与TNF联合对人髓性白血病细胞系诱导分化的影响。TGF-β 1单独诱导人单核细胞白血病U-937系分化为具有巨噬细胞特征的细胞。当与TNF组合时，TGF-β 1协同或相加地诱导分化相关特性。人髓性白血病细胞系ML-1对TGF-β 1诱导分化的反应不同。TGF-β 1抑制TNF诱导的FcR活性和吞噬活性。然而，硝基蓝四唑还原活性协同诱导的TGF-β 1和TNF的组合。对TNF受体的Scatchard分析表明，用TGF-β 1处理后，U-937或ML-1细胞上TNF受体的结合位点数和解离常数没有改变。尽管IFN-γ、IL-6、粒细胞CSF和粒细胞-巨噬细胞CSF诱导的硝基四氮唑降低U-937细胞的活性，但只有IFN-γ和TNF与TGF-β 1联合协同诱导。在抑制U-937和ML-1细胞的生长中也观察到TGF-β 1和TNF之间的协同作用。尽管TGF-β 1对其他单核细胞样白血病THP-1细胞的分化诱导与U-937细胞的分化诱导相似，但TGF-β 1单独或与TNF组合仅轻微诱导早幼粒细胞白血病HL-60细胞的分化。我们的观察表明，TGF-β 1强烈调节人髓性白血病细胞，巨噬细胞前体的分化和增殖。
Effects of transforming growth factor-beta 1 (TGF-beta 1), either alone or in combination with TNF, on the induction of differentiation of human myelogenous leukemic cell lines were examined. TGF-beta 1 alone induced differentiation of a human monocytic leukemia U-937 line into the cells with macrophage characteristics. When combined with TNF, TGF-beta 1 synergistically or additively induced differentiation associated properties. A human myeloblastic leukemia cell line, ML-1, differently responded to TGF-beta 1 in induction of differentiation. FcR activity and phagocytic activity induced by TNF were suppressed by TGF-beta 1. However, nitroblue tetrazolium reducing activity was synergistically induced by combinations of TGF-beta 1 and TNF. Scatchard analysis of TNF receptors indicated that the number of binding sites and dissociation constant of TNF for its receptors on U-937 or ML-1 cells were not changed by treatment with TGF-beta 1. Although IFN-gamma, IL-6, granulocyte CSF, and granulocyte-macrophage CSF-induced nitroblue tetrazolium reducing activity of U-937 cells, only IFN-gamma, and TNF induced it synergistically in combination with TGF-beta 1. Synergism between TGF-beta 1 and TNF was also observed in inhibition of growth of U-937 and ML-1 cells. Although TGF-beta 1 induction of differentiation of other monocytoid leukemic THP-1 cells was similar to that of U-937 cells, TGF-beta 1 only slightly induced differentiation of promyelocytic leukemic HL-60 cells, either alone or in combination with TNF. Our observations indicate that TGF-beta 1 strongly modulates differentiation and proliferation of human myelogenous leukemia cells, macrophage precursors.