Adhesion of normal and sickle erythrocytes to endothelial monolayer cultures.
Adhesion of normal and sickle erythrocytes to endothelial monolayer cultures.
复制标题
正常和镰状红细胞对内皮单层培养物的粘附。
作者：
R. Hoover;R. Rubin;G. Wise;R. Warren
通过实验验证了一种假设，即正常人和镰刀状红细胞患者的红细胞表面的差异反映在与毛细血管衬里的附着程度上。采用51Cr标记的红细胞(正常或镰刀状)附着于培养的小牛主动脉内皮细胞单层的方法。在此条件下，镰状细胞患者的红细胞与内皮细胞的粘附性好于正常患者。结果提示，镰状细胞与内皮细胞的黏附增强可能是导致镰状细胞贫血症状的毛细血管阻塞增加的部分原因。
Experiments were carried out to test the hypothesis that the differences between the surfaces of erythrocytes from normal and sickle cell patients are reflected in the degree of attachment to the capillary lining. An assay was used that measured the number of 51Cr-labeled erythrocytes (normal or sickle) attaching to a monolayer of endothelium cultured from calf aortas. Under these conditions, erythrocytes from sickle cell patients adhered better to the endothelium than did those from normal patients. The results suggested that the enhanced adhesion of the sickle cells to the endothelium may be partially responsible for the increased blockage of capillaries that produce the symptoms in sickle cell anemia.