A model for microtubule-associated protein 4 structure. Domains defined by comparisons of human, mouse, and bovine sequences.
A model for microtubule-associated protein 4 structure. Domains defined by comparisons of human, mouse, and bovine sequences.
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DOI：
10.1016/s0021-9258(18)54720-7
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发表时间：
1991-11
期刊：
影响因子：
--
通讯作者：
R. West;K. Tenbarge;J. B. Olmsted
中科院分区：
文献类型：
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作者：
R. West;K. Tenbarge;J. B. Olmsted
分离编码人和小鼠微管相关蛋白4 （MAP 4）的cdna。map4由一个基因编码。多种map4 mrna转录在小鼠组织中有差异表达。人类和小鼠MAP 4克隆的开放阅读框显示了三个不同的区域，由具有不同基序的相关序列组成。大约30%的蛋白质是大约14个氨基酸的串联相关重复序列。另一个区域包含丝氨酸和脯氨酸簇。map2和tau微管结合结构域的4个18-mer重复序列位于map4的羧基末端。氨基酸序列分析表明，人和小鼠的map4是牛190 kda MAP/ mapu的同源物（Aizawa， H., Emori， Y., Murofushi， H., Kawasakai， H., Sakai， H., and Suzuki， K., 1990）。化学，265,13849-13855)。小鼠和人的map4与牛的190-kDa的map4相似度约为75%，表明这些蛋白都是同一类的成员。具有极高保守性（大于或等于88%）的结构域有：1)氨基末端；2) KDM和S，P结构域之间存在脯氨酸富集区；3)微管结合域；4)羧基末端。
cDNAs encoding human and mouse microtubule-associated protein 4 (MAP 4) were isolated. MAP 4 is encoded by a single gene. Multiple MAP 4 mRNAs are transcribed that are differentially expressed among mouse tissues. Open reading frames for the human and mouse MAP 4 clones indicate three distinct regions consisting of related sequences with different motifs. Approximately 30% of the protein is tandem related repeats of approximately 14 amino acids. Another region contains clusters of serine and proline. Four 18-mer repeats characteristic of the microtubule-binding domains of MAP 2 and tau are located at the carboxyl-terminal portion of MAP 4. Amino acid sequence analysis revealed that human and mouse MAP 4 are homologs of the bovine 190-kDa MAP/MAP U (Aizawa, H., Emori, Y., Murofushi, H., Kawasakai, H., Sakai, H., and Suzuki, K. (1990) J. Biol. Chem. 265, 13849-13855). Mouse and human MAP 4 and the bovine 190-kDa MAP are approximately 75% similar, indicating that these proteins are all members of the same class. Domains with extremely high conservation (greater than or equal to 88%) are: 1) the extreme amino terminus; 2) a proline-rich region between the KDM and S,P domains; 3) the microtubule-binding domain; and 4) the extreme carboxyl terminus.