SURVEY OF INTERACTION OF CALCIUM IONS WITH MITOCHONDRIA FROM DIFFERENT TISSUES AND SPECIES
SURVEY OF INTERACTION OF CALCIUM IONS WITH MITOCHONDRIA FROM DIFFERENT TISSUES AND SPECIES
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DOI：
10.1042/bj1220681
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发表时间：
1971-01-01
影响因子：
4.1
通讯作者：
LEHNINGE.AL
中科院分区：
文献类型：
--
作者：
CARAFOLI, E;LEHNINGE.AL
从许多不同组织和物种中分离的线粒体在电子传递过程中从悬浮介质中积累Ca 2+的能力进行了调查。检查的种属包括大鼠、小鼠、兔、仓鼠、豚鼠、牛、鸡、龟、丽蝇、酵母和粗糙脉孢菌。检查的组织包括脊椎动物的肝脏、肾脏、大脑、心脏、脾脏、甲状腺和肾上腺皮质，以及绿头苍蝇的飞行肌肉。所有脊椎动物组织的线粒体检查表明：（a）刺激状态4呼吸的Ca 2 + Ca ~（2+）/~（2+）激活比约为2.0，伴随着Ca ~（2+）的积累和H ~（2+）的排出，H ~（2+）/Ca ~（2+）比值约为1.0，（B）大量Ca ~（2+）积累需要磷酸盐，（c）不依赖于呼吸的高亲合性Ca ~（2+）结合位点，（d）高亲合性Ca ~（2+）结合位点，（e）高亲合性Ca ~（2+）结合位点。（d）内源性Ca 2+，其主要由解偶联剂释放。然而，从酵母和苍蝇飞行肌肉的线粒体是无法积累的呼吸依赖性过程中的Ca 2+，并拥有没有高亲和力的Ca 2+结合位点。这些发现支持了这样的观点：高亲和力位点代表了膜中特定Ca 2+“渗透酶”或转运系统的配体结合位点。相对较高的亲和力的Ca 2+，这等于或超过ADP的亲和力，和一般均匀的特点，在所有的脊椎动物线粒体中的Ca 2+运输测试强烈表明，呼吸相关的Ca 2+积累在脊椎动物细胞生理学中起着普遍的和基本的作用。
A survey was made of the capacity of mitochondria isolated from a number of different tissues and species to accumulate Ca2+from the suspending medium during electron transport. The species examined included the rat, mouse, rabbit, hamster, guinea pig, cow, chicken, turtle, blowfly, yeast andNeurospora crassa. The tissues examined included vertebrate liver, kidney, brain, heart, spleen, thyroid and adrenal cortex, and the flight muscle of the blowfly. The mitochondria from all vertebrate tissues examined showed: (a) stimulation of State 4 respiration by added Ca2+(Ca2+/~ activation ratio about 2.0), accompanied by accumulation of Ca2+and ejection of H+, with a H+/Ca2+ratio about 1.0; (b) a requirement of phosphate for accumulation of large amounts of Ca2+; (c) respiration-independent high-affinity binding sites for Ca2+; (d) endogenous Ca2+, which is largely released by uncoupling agents. However, mitochondria from yeast and blowfly flight muscle are unable to accumulate Ca2+in a respiration-dependent process and possess no high-affinity Ca2+-binding sites. These findings support the view that the high-affinity sites represent the ligand-binding sites of a specific Ca2+‘permease’ or transport system in the membrane. The relatively high affinity for Ca2+, which equals or exceeds the affinity for ADP, and the generally uniform characteristics of Ca2+transport in all the vertebrate mitochondria tested strongly suggest that respiration-linked Ca2+accumulation plays a general and fundamental role in vertebrate cell physiology.