Ascorbic Acid Accumulation and Expression of Genes Involved in Its Biosynthesis and Recycling in Developing Apple Fruit
Ascorbic Acid Accumulation and Expression of Genes Involved in Its Biosynthesis and Recycling in Developing Apple Fruit
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苹果果实发育过程中抗坏血酸的积累及其生物合成和回收相关基因的表达
DOI：
10.21273/jashs.136.4.231
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发表时间：
2011-07-01
影响因子：
1.9
通讯作者：
Ma, Fengwang
中科院分区：
文献类型：
--
作者：
Li, Mingjun;Chen, Xuesen;Ma, Fengwang
本研究旨在研究苹果果实发育过程中抗坏血酸（AsA）积累、AsA合成与循环相关基因mRNA表达、关键酶活性及其与AsA水平的关系。联欢晚会)。AsA浓度在幼果开花后最高，随后逐渐下降，主要依赖于生物合成。然而，随着时间的推移，由于果实质量的增加，AsA继续积累。二磷酸鸟苷(GDP)- l -半乳糖磷酸化酶、GDP-甘露糖焦磷酸化酶、d -半乳糖还原酶和转录后调节的l -半乳糖-1,4-内酯脱氢酶的转录水平与AsA积累无关。相比之下，l -半乳糖脱氢酶、l -半乳糖-1-磷酸磷酸酶和gdp -甘露糖-3#、5#- epimase的表达模式与AsA积累的变化模式相似。AsA循环能力较弱的果实中单脱氢抗坏血酸还原酶和脱氢抗坏血酸还原酶的活性和表达量远低于叶片，但与果实发育过程中AsA水平的相关性不明显。
The objective of this study was to investigate ascorbic acid (AsA) accumulation, mRNA expression of genes involved in AsA biosynthesis as well as recycling, activity of key enzymes, and the relationship of them to AsA levels during the development of apple fruit (Malus ·domestica cv. Gala). AsA concentration, which mainly depends on biosynthesis, was the highest in young fruit post-anthesis and then decreased steadily toward maturation. However, AsA continued to accumulate over time because of the increase in fruit mass. Transcript levels of guanosine diphosphate (GDP)-L-galactose phosphorylase, GDP-mannose pyrophosphorylase, D-galacturonate reductase, and the post-transcriptionally regulated L-galactono-1,4-lactone dehydrogenase were not correlated with AsA accumu- lation in apple. In contrast, patterns of expression for L-galactose dehydrogenase, L-galactose-1-phosphate phosphatase, and GDP-mannose-3#,5#-epimerase showed a pattern of change similar to that of AsA accumulation. Although activities and expression levels of monodehydroascorbate reductase and dehydroascorbate reductase in fruit, which had less capacity for AsA recycling, were much lower than in leaves, they were not clearly correlated with AsA level during fruit development.