Triethyloxonium Tetrafluoroborate Derivatization and HPLC Analysis of Diethyldithiocarbamate in Plasma
Triethyloxonium Tetrafluoroborate Derivatization and HPLC Analysis of Diethyldithiocarbamate in Plasma
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三乙基氧鎓四氟硼酸盐衍生化及血浆中二乙基二硫代氨基甲酸酯的 HPLC 分析
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
R. Borch
中科院分区：
文献类型：
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作者：
P. Lieder;R. Borch
摘要建立了一种衍生化-高效液相色谱法定量测定血浆中二乙基二硫代氨基甲酸乙酯（DDTC）的方法。血浆样品通过在二氯甲烷中加入10ul Meerwein试剂（et30 +BF4−）快速衍生化。得到的s -乙基二硫代氨基甲酸乙酯被提取到含有s -异丙基二硫代氨基甲酸乙酯作为内标的氯仿中。采用254 nm反相高效液相色谱法测定DDTC的浓度。DDTC作为s -乙酯的检出限为5 ng/ml。在250 ~ 500002ng /ml范围内，色谱响应呈线性关系（R2 < 0.9995）。在接受顺-二胺二氯铂（II）（顺铂）化疗的患者临床使用DDTC作为肾脏拯救剂之前，该方法已被用于测定动物模型中DDTC的血浆水平。
Abstract An analytical method has been developed to quantitate diethyldithiocarbamate (DDTC) in plasma by derivatization and HPLC analysis. A plasma sample is rapidly derivatized by addition of 10 ul of Meerwein's reagent (Et3O+BF4 −) in methylene chloride. The resulting S-ethyl diethyldithiocarbamate is extracted into chloroform containing S-isopropyl diethyldithiocarbamate as the internal standard. Concentrations of DDTC are then determined by reverse phase HPLC analysis with detection at 254 nm. The detection limit for DDTC as the S-ethyl ester is 5 ng/ml. The chromatographic response is linear over the range of 250 to 500,000 2 ng/ml (R2 < 0.9995). This method has been used to determine DDTC plasma levels in animal models prior to clinical use of DDTC as a kidney rescue agent for patients receiving cis-diamminedichloroplatinum(II) (Cisplatin) chemotherapy.
DOI：
10.1073/pnas.77.9.5441
发表时间：
1980-09
影响因子：
11.1
作者：
R. Borch;J. Katz;P. Lieder;M. E. Pleasants
通讯作者：
R. Borch;J. Katz;P. Lieder;M. E. Pleasants