The effects of hyperbaric oxygen therapy on blood-brain barrier permeability in septic rats
The effects of hyperbaric oxygen therapy on blood-brain barrier permeability in septic rats
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DOI：
10.1016/j.brainres.2011.07.020
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发表时间：
2011-09-15
期刊：
影响因子：
2.9
通讯作者：
Ahishali, Bulent
中科院分区：
文献类型：
--
作者：
Autan, Sezen Milli;Kaya, Mehmet;Ahishali, Bulent
败血症脑病血脑屏障(BBB)完整性改变的机制尚不清楚。本研究旨在探讨高压氧治疗(HBOT)是否影响大鼠血脑屏障对盲肠结扎穿孔(CLP)所致脓毒症的反应。测定大脑皮层和海马区组织中肿瘤坏死因子-α(TNF-α)、丙二醛(MDA)和谷胱甘肽(GSH)水平。通过测定伊文思蓝(EB)和辣根过氧化物酶(HRP)示踪剂的渗出量，从功能和结构上评价血脑屏障的通透性。免疫组织化学和Western blotting检测封闭素的表达。高压氧治疗不改变CLP手术大鼠的肿瘤坏死因子-α水平，而对正常大鼠应用高压氧治疗后则显著降低。CLP加HBOT组动物的丙二醛水平显著降低。在脓毒症大鼠中，HBOT可显著提高降低的GSH水平。高压氧可减轻慢性阻塞性肺疾病大鼠血脑屏障对EB通透性的增加(P
The mechanisms underlying the changes in blood-brain barrier (BBB) integrity in septic encephalopathy are poorly understood. The present study was designed to examine whether hyperbaric oxygen therapy (HBOT) influences the response of BBB to sepsis induced by cecal ligation and puncture (CLP) in rats. Cerebral cortical and hippocampal tissue levels of tumor necrosis factor-alpha (TNF-alpha), malondialdehyde (MDA) and glutathione (GSH) levels were measured. BBB permeability was functionally and structurally evaluated by determining extravasation of Evans blue (EB) and horseradish peroxidase (HRP) tracers, respectively. Immunohistochemistry and western blotting for occludin were performed. HBOT did not alter TNF-alpha levels in CLP operated rats while a significant decrease was noted when the therapy was subjected to intact rats. MDA levels in animals subjected to CLP plus HBOT were significantly decreased. In septic rats, the decreased GSH levels were significantly increased by HBOT. While HBOT attenuated the increased BBB permeability to EB in rats subjected to CLP (P