Transgenic perennial ryegrass plants expressing wheat fructosyltransferase genes accumulate increased amounts of fructan and acquire increased tolerance on a cellular level to freezing
Transgenic perennial ryegrass plants expressing wheat fructosyltransferase genes accumulate increased amounts of fructan and acquire increased tolerance on a cellular level to freezing
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DOI：
10.1016/j.plantsci.2004.05.037
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发表时间：
2004-10-01
期刊：
影响因子：
5.2
通讯作者：
Yamada, T
中科院分区：
文献类型：
--
作者：
Hisano, H;Kanazawa, A;Yamada, T
秋季禾本科植物果聚糖的积累与其抗寒性有关。果聚糖积累的遗传调控是提高禾本科植物抗寒性的重要分子育种策略。我们生产的转基因多年生黑麦草（Lolium perenne）植物过表达小麦果糖基转移酶基因，wft 1和wft 2，分别编码蔗糖-果聚糖6-果糖基转移酶（6-SFT）和蔗糖-蔗糖1-果糖基转移酶（1-SST），在CaMV 35 S启动子的控制下，使用粒子轰击介导的转化方法。在转基因多年生黑麦草植株中检测到果聚糖含量的显著增加。使用电导率方法的冷冻试验表明，积累了比非转基因植物更大量果聚糖的转基因植物在细胞水平上对冷冻的耐受性增加。结果表明，过量表达参与果聚糖合成的基因可作为一种新的策略，以产生耐冷冻草。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
The accumulation of fructan in grasses during autumn is linked to winter hardiness. Genetic manipulation of the accumulation of fructan could be an important molecular breeding strategy for the improvement of winter hardiness in grasses. We produced transgenic perennial ryegrass (Lolium perenne) plants that overexpress wheat fructosyltransferase genes, wft1 and wft2, which encode sucrose-fructan 6-fructosyltransferase (6-SFT) and sucrose-sucrose 1-fructosyltransferase (1-SST), respectively, under the control of CaMV 35S promoter using a particle bombardment-mediated method of transformation. Significant increases in fructan content were detected in the transgenic perennial ryegrass plants. A freezing test using the electrical conductivity method indicated that transgenic plants that accumulated a greater amount of fructan than non-transgenic plants have increased tolerance on a cellular level to freezing. The results suggest that the overexpression of the genes involved in fructan synthesis serves as a novel strategy to produce freezing-tolerant grasses. (C) 2004 Elsevier Ireland Ltd. All rights reserved.