Tissue-specific knockout of androgen receptor in mice.
Tissue-specific knockout of androgen receptor in mice.
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DOI：
10.1007/978-1-61779-243-4_16
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发表时间：
2011
影响因子：
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通讯作者：
Tzu-hua Lin;S. Yeh;Chawnshang Chang
中科院分区：
文献类型：
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作者：
Tzu-hua Lin;S. Yeh;Chawnshang Chang
雄激素通过雄激素受体（AR）发挥作用，对男性性分化和发育至关重要。利用Cre-lox技术，我们已经产生了floxed AR小鼠，这些小鼠已经与一般或组织特异性表达Cre的转基因小鼠一起培育，以敲除特定靶细胞中的AR基因。我们的研究结果表明，AR是性发育所必需的，AR的缺失可能对生理功能和疾病进展的许多方面产生重大影响，如免疫功能，代谢和肿瘤发生。此外，我们的策略可以在雌性小鼠中产生AR敲除（ARKO），这使得研究人员能够研究雌性小鼠的AR功能。简而言之，我们的floxed AR小鼠模型提供了一个强大的工具，在体内研究AR功能的选择性组织和细胞类型，并可能在内分泌学领域的几个研究突破。
Androgen acting through the androgen receptor (AR) is known to be essential for male sexual differentiation and development. UsingCre-lox technology, we have generated the floxed AR mice, which have been bred with general or tissue-specificCreexpressing transgenic mice to knock out the AR gene in specific target cells. Our findings indicated that AR is required for sexual development and that loss of AR can have significant effects on many aspects of physiological functions and disease progression, such as immune function, metabolism, and tumorigenesis. Furthermore, our strategy can generate AR knockout (ARKO) in female mice, which allows researchers to study the AR function in the female. In brief, our floxed AR mouse model provides a powerful tool to study in vivo AR functions in selective tissues and cell types and has made possible several research breakthroughs in the field of endocrinology.