THE NCI-H295 CELL-LINE - A PLURIPOTENT MODEL FOR HUMAN ADRENOCORTICAL STUDIES
THE NCI-H295 CELL-LINE - A PLURIPOTENT MODEL FOR HUMAN ADRENOCORTICAL STUDIES
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DOI：
10.1016/0303-7207(94)90277-1
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发表时间：
1994-04-01
影响因子：
4.1
通讯作者：
MASON, JI
中科院分区：
文献类型：
--
作者：
RAINEY, WE;BIRD, IM;MASON, JI
人肾上腺皮质是一个复杂的内分泌器官，分泌矿物皮质激素、糖皮质激素和肾上腺雄激素。这些类固醇产生于肾上腺形态和生物化学上不同的区域。在过去，研究这些不同的类固醇激素的分泌需要从每个肾上腺皮质区分离细胞。事实上，缺乏能够产生任何主要肾上腺类固醇产物的人类肾上腺皮质细胞系已经减缓了对正常和异常肾上腺功能的研究。随着H295细胞的出现，这一障碍已被很大程度上克服。H295细胞是第一个在特定条件下保持产生所有肾上腺皮质类固醇（即矿物皮质激素、糖皮质激素和肾上腺雄激素）能力的肾上腺皮质细胞系。因此，H295细胞似乎作为多能性肾上腺皮质细胞，能够被引导产生每一种区域特异性类固醇。H295细胞系在肾上腺甾体形成的分子生化机制研究中具有重要的应用价值。
The human adrenal cortex is a complex endocrine organ that secretes mineralocorticoids, glucocorticoids and adrenal androgens. These steroids arise from morphologically and biochemically distinct zones of the adrenal gland. Studying secretion of these distinct steroid hormones has, in the past, required the isolation of cells from each of the adrenocortical zones. Indeed, the lack of a human adrenocortical cell line retaining the ability to produce any of the major adrenal steroid products has slowed studies on normal and abnormal adrenal function. This obstacle has now been largely overcome with the availability of H295 cells, which represents the first adrenocortical cell line to maintain the ability, under specified conditions, to produce all the adrenocortical steroids (i.e., mineralocorticoids, glucocorticoids, and adrenal androgens). Thus, H295 cells appear to act as pluripotent adrenocortical cells capable of being directed to produce each of the zone-specific steroids. The H295 cell line should prove to be of value in studying the molecular and biochemical mechanisms controlling adrenal steroidogenesis.