Photoacoustic Imaging for Lymphatic Vein Anastomosis ? Examination using small animals
Photoacoustic Imaging for Lymphatic Vein Anastomosis ? Examination using small animals
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光声成像用于淋巴静脉吻合术？
DOI：
10.1109/ultsym.2019.8926173
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Shiina Tsuyoshi
中科院分区：
文献类型：
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作者：
Sakata Moe;Yamakawa Makoto;Namita Takeshi;Kondo Kengo;Shiina Tsuyoshi
淋巴管及其周围血管的直径和形状为确定水肿进展程度和随后的淋巴静脉吻合计划提供了重要信息。该项目的目的是利用光声成像（派）在三维空间中显示这些信息。在这项研究中，我们使用了半球形传感器阵列派系统，它提供了高分辨率和宽视场，并显示3D图像。我们用吲哚菁绿色（ICG）来检测派。由于氧合血红蛋白、脱氧血红蛋白和ICG具有不同的光学吸收光谱，因此我们能够通过使用两种波长（800 nm和850 nm）来区分淋巴管和血管。我们的派系统通过使用半球形传感器阵列执行2D扫描来执行3D重建。我们将ICG注入小鼠和大鼠的手指，并对整个足部的淋巴管和血管进行成像。在进行光声测量之后，我们使用解剖和ICG荧光成像来确认淋巴管和血管的直径和形状。对于大鼠的测量，含有ICG的淋巴管在血管的两侧可见。在较小小鼠的派中，淋巴结与淋巴管一起沿着显示。利用派很容易区分淋巴管和血管，并且我们能够在三维中显示淋巴管和血管。
The diameters and shapes of lymphatic vessels and the blood vessels which surround them provide important information used in determining the degree of progression of lymphedema and the subsequent planning of lymphatic vein anastomosis. The aim of this project is to display this information in three dimensions using photoacoustic imaging (PAI). For this study, we used a hemispherical sensor array PAI system that provides both a high resolution and a wide field of view and displays a 3D image. We used indocyanine green (ICG) to detect lymphatics using PAI. Because oxyhemoglobin, deoxyhemoglobin, and ICG have different optical absorption spectra, we were able to distinguish between lymphatic vessels and blood vessels by using two wavelengths (800 nm and 850 nm). Our PAI system performed 3D reconstruction by performing 2D scanning with a hemispherical sensor array. We injected ICG into mouse and rat fingers and imaged the lymphatic and blood vessels of the entire foot. After performing photoacoustic measurements, we confirmed the diameter and shape of lymphatic and blood vessels using dissection and ICG fluorescence imaging. For measurements of rats, lymphatic vessels containing ICG are visible on both sides of the blood vessel. In PAI of smaller mice, lymph nodes are displayed along with lymphatic vessels. It is easy to distinguish lymphatic vessels and blood vessels using PAI, and we were able to demonstrate lymphatic and blood vessels in three dimensions.