Fetal alcohol exposure alters serotonin transporter sites in rat brain.
Fetal alcohol exposure alters serotonin transporter sites in rat brain.
复制标题
胎儿酒精暴露会改变大鼠大脑中的血清素转运蛋白位点。
DOI：
10.1016/s0006-8993(99)02350-1
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发表时间：
2000
期刊：
影响因子：
2.9
通讯作者：
Tejani-Butt,S
中科院分区：
文献类型：
--
作者：
Zafar,H;Shelat,SG;Redei,E;Tejani-Butt,S
本研究采用定量放射自显影技术研究了胎儿酒精暴露（FAE）对发育中的雄性和雌性大鼠子代脑中5-羟色胺转运体（5-HTT）结合位点的影响。给妊娠时间的母鼠喂食液体乙醇饮食、不含乙醇的等热量饮食或正常大鼠食物。在21、40和60日龄时处死雄性和雌性后代，取出大脑并切片以分析5-HTT位点。FAE导致不同的影响5-HTT网站取决于年龄和性别的后代。FAE增加5-HTT结合位点在皮质层5，6，海马CA 2，3，杏仁核外侧核和中缝背核。FAE使杏仁核内侧核、下丘脑背内侧核和腹内侧核的5-HTT结合位点减少。FAE降低5-HTT结合位点暂时在腹内侧核的下丘脑在21日龄的女性，这种影响被发现在第40天消失。与此相反，FAE增加5-HTT网站在杏仁核外侧核的成年动物，表明乙醇暴露在子宫内可能会改变5-羟色胺的神经传递在离散的大脑区域永久。
This study examined the effects of fetal alcohol exposure (FAE) on serotonin transporter (5-HTT) binding sites in the brains of developing male and female rat offspring using the technique of quantitative autoradiography. Time-pregnant dams were fed liquid ethanol diet, isocaloric diet without ethanol or normal rat chow. Male and female offspring were sacrificed at 21, 40 and 60 days of age, brains removed and sectioned for analysis of 5-HTT sites. FAE led to distinct effects on 5-HTT sites depending on the age and gender of the offspring. FAE increased 5-HTT binding sites in cortical layers 5, 6, hippocampal layers CA2,3, lateral nucleus of the amygdala and in the dorsal raphe nucleus. FAE decreased 5-HTT binding sites in the medial nucleus of amygdala, dorsomedial and ventromedial nuclei of the hypothalamus. FAE decreased 5-HTT binding sites temporarily in the ventromedial nucleus of the hypothalamus in the 21-day-old female; this effect was found to disappear by day 40. In contrast, FAE increased 5-HTT sites in the lateral nucleus of the amygdala in the adult animal, suggesting that ethanol exposure in utero may alter serotonin neurotransmission in discrete brain regions permanently.