Inhibitory effects of xylitol and sorbitol on Streptococcus mutans and Candida albicans biofilms are repressed by the presence of sucrose
Inhibitory effects of xylitol and sorbitol on Streptococcus mutans and Candida albicans biofilms are repressed by the presence of sucrose
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DOI：
10.1016/j.archoralbio.2020.104886
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发表时间：
2020-11-01
影响因子：
3
通讯作者：
Seneviratne, Chaminda Jayampath
中科院分区：
文献类型：
--
作者：
Chan, Ally;Ellepola, Kassapa;Seneviratne, Chaminda Jayampath
目的：在现有的预防和治疗龋齿的方法中，糖醇(木糖醇和山梨醇)作为口腔保健品被广泛推广，因为它具有明显的防龋性。然而，这些糖醇在添加蔗糖的环境中对变形链球菌和白色念珠菌的治疗效果还没有得到充分的研究，因为在口腔易患疾病的情况下，这些糖醇对变形链球菌和白色念珠菌的治疗效果还没有得到充分的研究。方法：在含有不同浓度的木糖醇、山梨醇和添加或不添加蔗糖的培养基中，评价单种和混合种生物膜的形成。结果：在无蔗糖条件下，木糖醇和山梨醇对变形链球菌生物膜有明显的抑制作用，而对白色念珠菌生物膜的抑制作用较弱。环境中1%蔗糖的存在减弱了木糖醇和山梨醇对变形链球菌和白色念珠菌单种生物膜的抑制作用。在预先形成的变形链球菌生物膜上添加蔗糖也降低了木糖醇的抑制作用。木糖醇和山梨醇的存在减少了混合生物膜的形成，并改变了生物膜的结构和葡聚糖的生产。结论：木糖醇和山梨醇对变形链球菌和白色念珠菌的生物被膜形成有抑制作用，且这种抑制作用可被蔗糖所抑制。
Objective: Among the preventive and therapeutic options available for dental caries, sugar alcohols (xylitol and sorbitol) have been widely promoted as oral healthcare products due to its perceived anticariogenic effect. However, the therapeutic efficacy of these sugar alcohols against Streptococcus mutans and Candida albicans in a sucrose supplemented environment, as found in disease-prone conditions in the oral cavity, has not been adequately investigated.Methods: Single and mixed-species biofilm formation was evaluated in medium with different concentrations of xylitol, sorbitol with or without sucrose supplementation. Biofilm quantification methods such as crystal violet assay, XTT assay, CFU counting complemented with confocal and electron microscopic techniques were used.Results: Under sucrose-free conditions, xylitol and sorbitol demonstrated a significant dose-dependent inhibitory effect on S. mutans biofilms, whereas inhibitory effect on C. albicans biofilm was weak. The presence of 1 % sucrose in the environment diminished the inhibitory effect of both xylitol and sorbitol on S. mutans and C. albicans mono-species biofilms. Sucrose supplementation on pre-formed S. mutans biofilms also reduced the inhibitory effect of xylitol. Xylitol and sorbitol presence reduced mixed-species biofilm formation and altered the biofilm architecture and glucan production. However, sucrose supplementation reduced the inhibitory effect of sugar alcohols and enhanced the mixed-species biofilm formation.Conclusions: Xylitol and sorbitol exerts an inhibitory effect on S. mutans and C. albicans biofilm formation and this inhibitory effect is repressed by the presence of sucrose.