Nitric oxide and noradrenaline contribute to the temperature threshold of the axon reflex response to gradual local heating in human skin
Nitric oxide and noradrenaline contribute to the temperature threshold of the axon reflex response to gradual local heating in human skin
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DOI：
10.1113/jphysiol.2005.104067
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发表时间：
2006-05-01
影响因子：
5.5
通讯作者：
Minson, Christopher T.
中科院分区：
文献类型：
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作者：
Houghton, Belinda L.;Meendering, Jessica R.;Minson, Christopher T.
皮肤血流对局部快速加热的初始反应是轴突反射，其可能由降钙素基因相关肽和C纤维释放的P物质介导。我们调查的作用，一氧化氮（NO）和去甲肾上腺素的温度阈值的轴突反射逐渐局部加热过程中。36名受试者参加了两项研究。使用微透析，我们研究了以下干预措施：NO合成酶抑制（10 mM N-G-硝基-L-精氨酸甲酯，L-NAME）;低剂量NO输注（1.0 μ m硝普钠，SNP）;肾上腺素能阻断（10 mM甲苯磺酸溴苄铵）;和低剂量（0.1 μ m）去甲肾上腺素输注。采用激光多普勒血流仪测量红细胞流量。将皮肤以0.1 ℃ min（-1）的速率从33 ℃加热至40 ℃。与对照皮肤部位相比，轴突反射反应在4名受试者的L-NAME部位转移到更高的温度（对照，37.0 +/- 0.3摄氏度，n = 16; L-NAME，39.8 +/- 0.1摄氏度，n = 4; P < 0.001），在12名受试者中不存在。在L-NAME加低剂量SNP位点中也不存在这种反应，并且单独输注低剂量SNP也不改变这种反应。肾上腺素能阻滞，与或不与低剂量去甲肾上腺素输注，也取消了轴突反射反应在所有科目。与对照部位相比，低剂量去甲肾上腺素单独输注将轴突反射转移到显著较低的温度阈值（对照，38.2 +/- 0.5 ℃;去甲肾上腺素，37.7 +/- 0.4 ℃，P < 0.05，n = 5）。这些结果表明，内源性NO和去甲肾上腺素有助于在逐渐局部加热的皮肤轴突反射反应的温度阈值。
The initial skin blood flow response to rapid local heating is an axon reflex, which may be mediated by calcitonin gene-related peptide and substance P released from C-fibres. We investigated the role of nitric oxide (NO) and noradrenaline on the temperature threshold for the axon reflex during gradual local heating. 36 subjects participated in two studies. Using microdialysis, we examined the following interventions: NO synthase inhibition (10 mM N-G-nitro-L-arginine methyl ester, L-NAME); low-dose NO infusion (1.0 mu m sodium nitroprusside, SNP); adrenergic blockade (10 mM bretylium tosylate); and low-dose (0.1 mu m) noradrenaline infusion. Laser-Doppler flowmetry was used to measure red blood cell flux. Skin was heated at a rate of 0.1 degrees C min(-1) from 33 degrees C to 40 degrees C. Compared to control skin sites, the axon reflex response was shifted to a higher temperature in 4 subjects in the L-NAME sites (control, 37.0 +/- 0.3 degrees C, n = 16; L-NAME, 39.8 +/- 0.1 degrees C, n = 4; P < 0.001) and absent in 12 subjects. The response was also absent in L-NAME plus low-dose SNP sites and not altered by low-dose SNP infusion alone. Adrenergic blockade, with and without low-dose noradrenaline infusion, also abolished the axon reflex response in all subjects. Low-dose noradrenaline infusion alone shifted the axon reflex to a significantly lower temperature threshold compared to control sites (control, 38.2 +/- 0.5 degrees C; noradrenaline, 37.7 +/- 0.4 degrees C, P < 0.05, n = 5). These results suggest that endogenous; NO and noradrenaline contribute to the temperature threshold of the axon reflex response during gradual local heating of the skin.