Cloning and mapping of resistance gene homologues in melon
Cloning and mapping of resistance gene homologues in melon
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DOI：
10.1016/s0168-9452(01)00403-4
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发表时间：
2001-06-01
期刊：
影响因子：
5.2
通讯作者：
Arús, P
中科院分区：
文献类型：
--
作者：
Garcia-Mas, J;van Leeuwen, H;Arús, P
使用根据已知抗病基因的两个保守区域设计的简并引物，在甜瓜中获得了四个含有核苷酸结合位点（NBS）基序的抗性基因同源物家族。这四种同源物与其他物种中发现的含有 NBS 的抗性基因具有高度的氨基酸同一性。其中三个同源物（MRGH4、MRGH21 和 MRGH63）被放置在甜瓜的遗传图谱中。用 MRGH21 检测到的 RFLP 位于连锁群 7 的远端部分。同源物 MRGH63 和 MRGH4 检测到映射到连锁群 4 的 RFLP。这些位点相对于甜瓜中定位的抗性基因的图位位置以及这些位点在标记辅助选择和基于图位的克隆策略中的潜在用途进行了讨论。 (C) 2001 Elsevier Science Ireland Ltd. 保留所有权利。
Four families of resistance gene homologues containing the nucleotide binding site (NBS) motif were obtained in Cucumis melo using degenerate primers designed from two conserved regions of known disease resistance genes. The four homologues had high levels of amino acid identity to NBS-containing resistance genes found in other species. Three of these homologues (MRGH4, MRGH21 and MRGH63) were placed in the genetic map of melon. The RFLP detected with MRGH21 was located on the distal part of Linkage Group 7. Homologues MRGH63 and MRGH4 detected RFLPs that mapped to Linkage Group 4. The map positions of these loci relative to those of resistance genes mapped in melon and the potential use of these loci in marker-assisted selection and map-based cloning strategies is discussed. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.