Regulation of the heme A biosynthetic pathway: differential regulation of heme A synthase and heme O synthase in Saccharomyces cerevisiae.
Regulation of the heme A biosynthetic pathway: differential regulation of heme A synthase and heme O synthase in Saccharomyces cerevisiae.
复制标题
血红素 A 生物合成途径的调节：酿酒酵母中血红素 A 合酶和血红素 O 合酶的差异调节。
DOI：
10.1074/jbc.m804167200
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Hegg,EricL
中科院分区：
文献类型：
--
作者：
Wang,Zhihong;Wang,Yuxin;Hegg,EricL
细胞色素氧化酶的组装和活性依赖于其必需辅因子之一血红素A的可用性。在真核生物中，血红素A的生物合成需要两种线粒体内膜蛋白，血红素O合酶（Cox 10）和血红素A合酶（Cox 15）。在这份报告中，我们证明，在酵母COX 15的转录调控Hap 1，转录因子的活性是积极控制细胞内血红素浓度。相反，COX 10，COX 15的生理伴侣，不共享相同的调控机制与COX 15。有趣的是，蛋白质定量鉴定了Cox 15和Cox 10之间的8：1蛋白质比率。总之，这些结果表明，血红素A合酶和/或血红素O合酶可能发挥一个新的，身份不明的作用，除了血红素A的生物合成。
The assembly and activity of cytochromecoxidase is dependent on the availability of heme A, one of its essential cofactors. In eukaryotes, two inner mitochondrial membrane proteins, heme O synthase (Cox10) and heme A synthase (Cox15), are required for heme A biosynthesis. In this report, we demonstrate that inSaccharomyces cerevisiaethe transcription ofCOX15is regulated by Hap1, a transcription factor whose activity is positively controlled by intracellular heme concentration. Conversely,COX10, the physiological partner ofCOX15, does not share the same regulatory mechanism withCOX15. Interestingly, protein quantification identified an 8:1 protein ratio between Cox15 and Cox10. Together, these results suggest that heme A synthase and/or heme O synthase might play a new, unidentified role in addition to heme A biosynthesis.