Scutellariae radix Induces Apoptosis in Chemoresistant SCC-25 Human Tongue Squamous Carcinoma Cells
Scutellariae radix Induces Apoptosis in Chemoresistant SCC-25 Human Tongue Squamous Carcinoma Cells
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DOI：
10.1142/s0192415x15500111
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发表时间：
2015-01-01
影响因子：
5.7
通讯作者：
Kim, Gyoo-Cheon
中科院分区：
文献类型：
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作者：
Choi, Byul-Bora;Choi, Jeong Hae;Kim, Gyoo-Cheon
黄芩是韩国、日本、中国等亚洲国家使用最广泛的抗癌中草药之一。鳞状细胞癌(SCC)是最常见的头颈部肿瘤之一，具有高度侵袭性和转移性，并可能产生化疗耐药。因此，迫切需要新的有效的治疗方法。采用WST-1比色法、F-肌动蛋白染色、流式细胞仪分析、免疫荧光染色和Western印迹分析等方法检测黄芩对SCC-25细胞的作用。黄芩以剂量和时间依赖的方式抑制SCC-25细胞的生长，但不抑制人角质形成细胞的生长。观察细胞形态变化和丝状(F)-肌动蛋白结构的破坏。细胞色素c和凋亡诱导因子(AIF)移位到细胞核和胞浆中，表明黄芩可诱导细胞凋亡。黄芩诱导亚G1期细胞DNA含量增加，Bax、裂解caspase-3、caspase-7、caspase-9、DNA碎裂因子45(DFF45)和多聚(ADP-核糖)聚合酶-1(PARP-1)表达增加。此外，还检测到磷酸化丝裂原活化蛋白激酶(MAPK)相关蛋白的表达增加。黄芩的抗肿瘤作用是通过抑制细胞增殖、改变细胞形态、激活caspase和MAPK信号通路来实现的。综上所述，本研究结果突出了黄芩对耐药口腔鳞癌SCC-25细胞的抗癌活性。
Scutellariae radix is one of the most widely used anticancer herbal medicines in several Asian countries, including Korea, Japan, and China. Squamous cell carcinoma (SCC) is one of the most common head and neck carcinomas, which is highly invasive and metastatic, and can potentially develop chemoresistance. Therefore, new effective treatment methods are urgently needed. We determined the effects of Scutellariae radix on SCC-25 cells using the WST-1 assay, F-actin staining, flow cytometry analysis, immunofluorescence staining, and western blot analysis. Scutellariae radix treatment inhibited SCC-25 cell growth in a dose-and time-dependent manner, but it did not inhibit HaCaT (human keratinocyte) cell growth. Changes in cell morphology and disruption of filamentous (F)-actin organization were observed. Scutellariae radix-induced apoptosis as indicated by the translocation of cytochrome c and apoptosis-inducing factor (AIF) into the nucleus and cytosol. Scutellariae radix-induced an increase in cells with sub-G1 DNA content, and increased Bax, cleaved caspase-3, caspase-7, caspase-9, DNA fragmentation factor 45 (DFF 45), and poly(ADP-ribose) polymerase-1 (PARP-1) expression levels. Furthermore, increased expression of phosphorylated mitogen-activated protein kinase (MAPK)-related proteins was detected. The antitumor effect of Scutellariae radix was due to decreased cell proliferation, changes in cell morphology, and the activation of caspase and MAPK pathways. Taken together, the findings of this study highlight the anticancer activity of Scutellariae radix in chemoresistant SCC-25 oral squamous carcinoma cells.