Isolation of biogenetically competent mitochondria from mammalian tissues and cultured cells
Isolation of biogenetically competent mitochondria from mammalian tissues and cultured cells
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DOI：
10.1016/s1046-2023(02)00034-8
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发表时间：
2002-04-01
期刊：
影响因子：
4.8
通讯作者：
Enriquez, JA
中科院分区：
文献类型：
--
作者：
Fernández-Vizarra, E;López-Pérez, MJ;Enriquez, JA
本文介绍了一种适用于从不同来源纯化哺乳动物线粒体的快速基本方法。使用该方案获得的细胞器适用于生物遗传活性的研究，如酶活性，mtDNA，mtDNA，线粒体蛋白质合成，和线粒体tRNA氨酰化。此外，这些线粒体能够有效地输入蛋白质，并且通过DNA足迹法研究mtDNA/蛋白质相互作用也是可能的。(C)2002 Elsevier Science（美国）。All rights reserved.
This article describes a quick basic method adapted for the purification of mammalian mitochondria from different sources. The organelles obtained using this protocol are suitable for the investigation of biogenetic activities such as enzyme activity, mtDNA, mtRNA, mitochondrial protein synthesis, and mitochondrial tRNA aminoacylation. In addition, these mitochondria are capable of efficient protein import and the investigation of mtDNA/protein interactions by DNA footprinting is also possible. (C) 2002 Elsevier Science (USA). All rights reserved.