Optimization of a magnetic bead-based assay (MAGPIX(®)-Luminex) for immune surveillance of exposure to malaria using multiple Plasmodium antigens and sera from different endemic settings.
Optimization of a magnetic bead-based assay (MAGPIX(®)-Luminex) for immune surveillance of exposure to malaria using multiple Plasmodium antigens and sera from different endemic settings.
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利用来自不同地方性环境的多种疟原虫抗原和血清，优化了基于磁珠的测定法（Magpix（®）-luminex），以对疟疾的暴露进行免疫监测。
DOI：
10.1186/s12936-018-2465-4
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发表时间：
2018-09-06
期刊：
影响因子：
3
通讯作者：
Perraut R
中科院分区：
文献类型：
--
作者：
Varela ML;Mbengue B;Basse A;Loucoubar C;Vigan-Womas I;Dièye A;Toure A;Perraut R
血清学标记物是监测疟疾控制计划进展的潜在有用工具，但更好地了解抗体反应动力学是必要的。与ELISA相比，使用基于磁珠的免疫测定法（MBA）是有利的，因为它具有多重能力，但关于该测定法的标准化和验证的信息有限。使用一组4种抗原和98份来自塞内加尔农村无症状和城市有症状个体的血清，分析了疟原虫抗原抗体多重检测的几个参数。4种抗原包括恶性疟原虫CSP和PfAMA 1肽、重组恶性疟原虫MSP 4p 20和三日疟原虫CSP（PmCSP）肽。使用MSP 4p 20和全抗原提取物（SE）抗原进行与ELISA的比较。使用较少的微珠（每孔1000个微珠，而不是2000个微珠）和每106个微珠5 µg抗原被验证为较低的量。当使用肽时，载体蛋白（BSA）的使用被证明是关键的，并且评估了24小时延迟测量的效果（5-25%信号降低）。对抗体应答的分析表明，在所有传播环境中，抗体水平和流行率几乎同样高。使用PmCSP和SE抗原发现农村和城市疟疾之间存在明显区别。这项研究强调了进一步优化MBA技术的重要性，并强调了使用多阶段/多物种抗原在地方性环境中监测疟疾的兴趣。
Serological markers are potentially useful tools for monitoring the progress of malaria control programs, but a better understanding of antibody response dynamics is necessary. The use of a magnetic bead-based immunoassay (MBA) is advantageous compared to ELISA, due to its multiplexing capacity, but limited information is available on the standardization and validation of this assay. Several parameters for multiplex testing of antibodies to Plasmodium antigens were analysed using a set of 4 antigens and 98 sera from Senegalese rural asymptomatic and urban symptomatic individuals. The 4 antigens included Plasmodium falciparum CSP and PfAMA1 peptides, recombinant P. falciparum MSP4p20 and a Plasmodium malariae CSP (PmCSP) peptide. Comparisons with ELISA were done using MSP4p20 and whole schizont extract (SE) antigens. The use of fewer beads (1000 beads per well instead of 2000) and 5 µg of antigen per 106 bead were validated as lower amounts. The use of a carrier protein (BSA) was shown to be critical when using peptides and the effect of a 24 h delayed measures was evaluated (5–25% signal decrease). Analysis of Ab responses showed almost equally high levels and prevalence in all transmission settings. Clear distinctions between rural and urban malaria were noted using PmCSP and SE antigens. This study underlines the importance of further optimization of the MBA technique and highlights the interest of using multistage/multispecies antigens for surveillance of malaria in endemic settings.
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影响因子：
3
作者：
Baidjoe A;Stone W;Ploemen I;Shagari S;Grignard L;Osoti V;Makori E;Stevenson J;Kariuki S;Sutherland C;Sauerwein R;Cox J;Drakeley C;Bousema T
通讯作者：
Bousema T
影响因子：
17.1
作者：
Osier FH;Mackinnon MJ;Crosnier C;Fegan G;Kamuyu G;Wanaguru M;Ogada E;McDade B;Rayner JC;Wright GJ;Marsh K
通讯作者：
Marsh K
影响因子：
3
作者：
Diop, Fode;Richard, Vincent;Balde, Aissatou Toure
通讯作者：
Balde, Aissatou Toure
影响因子：
3
作者：
Badu, Kingsley;Siangla, Joram;Yan, Guiyun
通讯作者：
Yan, Guiyun
影响因子：
3.7
作者：
Cook, Jackie;Kleinschmidt, Immo;Drakeley, Chris J.
通讯作者：
Drakeley, Chris J.