PRESENCE OF INTACT INTERCELLULAR LIPID LAMELLAE IN THE UPPER LAYERS OF THE STRATUM-CORNEUM
PRESENCE OF INTACT INTERCELLULAR LIPID LAMELLAE IN THE UPPER LAYERS OF THE STRATUM-CORNEUM
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DOI：
10.1111/1523-1747.ep12470386
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发表时间：
1987-06-01
影响因子：
6.5
通讯作者：
DOWNING, DT
中科院分区：
文献类型：
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作者：
MADISON, KC;SWARTZENDRUBER, DC;DOWNING, DT
陆地生命所必需的表皮渗透屏障存在于角质层的细胞间隙中，由脂质组成。膜包被颗粒（MCG）是在活表皮的最上层发现的小的细胞内细胞器，其包含膜盘的堆叠，膜盘被挤压到细胞间隙中并经历生化和物理变化以形成构成该屏障的脂质片。使用四氧化钌作为第二固定剂，我们能够证明在角质层的最上层中填充细胞间隙的薄层堆叠。这些脂质层的结构与所提出的MCG脂质盘的衍生物一致，并且还表明与角质形成细胞包膜相邻的脂质双层可以由非衍生自MCG的脂质组装。
The epidermal permeability barrier necessary for terrestrial life resides in the intercellular spaces of the stratum corneum and is composed of lipids. Membrane coating granules (MCGs), small intracellular organelles found in the uppermost layers of the living epidermis, contain stacks of membranous disks which are extruded into the intercellular space and undergo both biochemical and physical changes to form the lipid sheets which constitute this barrier. Using ruthenium tetroxide as a secondary fixative, we are able to demonstrate stacks of lamellae filling the intercellular spaces in the uppermost layers of the stratum corneum. The structure of these lipid lamellae is consistent with the proposed derivation of MCG lipid disks and also suggests that the lipid bilayer adjacent to the corneocyte cell envelope may be assembled from lipids not derived from MCGs.