Bone Marrow-derived Endothelial Progenitor Cells and Endothelial Cells May Contribute to Endothelial Repair in the Kidney Immediately After Ischemia-Reperfusion
Bone Marrow-derived Endothelial Progenitor Cells and Endothelial Cells May Contribute to Endothelial Repair in the Kidney Immediately After Ischemia-Reperfusion
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DOI：
10.1369/jhc.2010.956011
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发表时间：
2010-08-01
影响因子：
3.2
通讯作者：
Uemura, Tadahiro
中科院分区：
文献类型：
--
作者：
Kwon, Osun;Miller, Shane;Uemura, Tadahiro
缺血性急性肾损伤时，肾血流量减少。我们之前的研究表明，再灌注、移植的肾脏表现出对血管内皮的缺血性损伤，而保持小管周围毛细血管内皮的完整性可能是缺血性损伤恢复的关键。我们假设骨髓源性内皮祖细胞（EPCs）可能在缺血后肾功能恢复中发挥重要作用。我们在移植前和移植后2周的尸体肾移植受者中验证了这一假设。我们发现循环中cd34阳性EPCs和cd146阳性内皮细胞（ECs）的数量在缺血再灌注后立即减少。在再灌注1小时后获得的同种异体肾移植组织中，与非缺血对照组相比，沿小管周围毛细血管内皮层观察到cd34阳性细胞的频率更高。此外，0-17.5%的小管周围毛细血管内皮细胞为受体来源。相比之下，只有0.1-0.7%的小管细胞来自受体。移植后35天和73天的重复移植活检样本不含受体来源的毛细血管内皮细胞，而分别有1.4%和12.1%的小管细胞是受体来源的。这些发现表明，BMD内皮祖细胞和内皮细胞可能有助于缺血再灌注后内皮细胞的立即修复。[J] .中国生物医学工程学报，2010。
In ischemic acute kidney injury, renal blood flow is decreased. We have previously shown that reperfused, transplanted kidneys exhibited ischemic injury to vascular endothelium and that preservation of peritubular capillary endothelial integrity may be critical to recovery from ischemic injury. We hypothesized that bone marrow derived (BMD) endothelial progenitor cells (EPCs) might play an important role in renal functional recovery after ischemia. We tested this hypothesis in recipients of cadaveric renal allografts before and for 2 weeks after transplantation. We found that the numbers of circulating CD34-positive EPCs and CD146-positive endothelial cells (ECs) decreased immediately after ischemia-reperfusion. In renal allograft tissues obtained 1 hr after reperfusion, CD34-positive cells were more frequently observed along the endothelial lining of peritubular capillaries compared with non-ischemic controls. Moreover, 0-17.5% of peritubular capillary ECs were of recipient origin. In contrast, only 0.1-0.7% of tubule cells were of recipient origin. Repeat graft biopsy samples obtained 35 and 73 days after transplant did not contain capillary ECs of recipient origin, whereas 1.4% and 12.1% of tubule cells, respectively, were of recipient origin. These findings suggest that BMD EPCs and ECs may contribute to endothelial repair immediately after ischemia-reperfusion. (J Histochem Cytochem 58:687-694, 2010)