Intracellular application of guanosine-5'-O-(3-thiotriphosphate) induces exocytotic granule fusion in guinea pig eosinophils.
Intracellular application of guanosine-5'-O-(3-thiotriphosphate) induces exocytotic granule fusion in guinea pig eosinophils.
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DOI：
10.1084/jem.171.3.775
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发表时间：
1990-03-01
期刊：
影响因子：
--
通讯作者：
Gomperts BD
中科院分区：
文献类型：
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作者：
Nüsse O;Lindau M;Cromwell O;Kay AB;Gomperts BD
用链溶素O通透性细胞悬液(纯度大于98%)释放己糖苷酶和全细胞膜片钳电容测定嗜酸性粒细胞分泌嗜酸性粒细胞的机制。结果表明，嗜酸性粒细胞成分的释放是通过单个颗粒与质膜融合的胞吐机制进行的。胞吐作用可通过细胞内应用非水解性鸟苷类似物鸟苷-5‘-O-(3-硫代三磷酸)(GTP-γ-S)来诱导，提示GTP结合蛋白参与了胞吐作用。这种激活受细胞内钙离子浓度的调节，被天冬氨酸-γ-S激活后引起细胞内钙离子浓度的一过性升高。因此，释放机制的性质和调节似乎与肥大细胞和中性粒细胞非常相似。
The mechanism of eosinophil secretion was studied in guinea pig eosinophils by measuring release of hexosaminidase from cell suspensions (greater than 98% pure) permeabilized with streptolysin-O and by whole-cell patch-clamp capacitance measurements. It is shown that release of eosinophil granule components occurs by an exocytotic mechanism in which individual granules fuse with the plasma membrane. Exocytosis can be induced by intracellular application of the nonhydrolyzable GTP analog guanosine-5'-O-(3-thiotriphosphate) (GTP- gamma-S), suggesting the involvement of a GTP-binding protein. The activation is modulated by the intracellular calcium concentration, with activation by GTP-gamma-S inducing transient elevations in the concentration of Ca2+. Thus, the nature and regulation of the release mechanism appear to be very similar to that of the mast cell and neutrophil.