Progression from papilloma to carcinoma is accompanied by changes in antibody response to papillomavirus proteins.
Progression from papilloma to carcinoma is accompanied by changes in antibody response to papillomavirus proteins.
复制标题
从乳头状瘤到癌的进展伴随着对乳头状瘤病毒蛋白的抗体反应的变化。
DOI：
10.1128/jvi.67.1.382-389.1993
复制
发表时间：
1993
影响因子：
5.4
通讯作者：
Wettstein,FO
中科院分区：
文献类型：
--
作者：
Lin,YL;Borenstein,LA;Selvakumar,R;Ahmed,R;Wettstein,FO
棉尾兔乳头瘤病毒可诱发兔的良性肿瘤、乳头状瘤，并以高频率进展为恶性肿瘤、癌。因此，棉尾兔乳头瘤病毒为致癌性人乳头瘤病毒提供了一个实验模型。在肿瘤发展的任何阶段，宿主识别的抗原的性质都没有被确定。在这里，我们描述了棉尾兔和乳头状瘤阶段的家兔、癌症阶段的家兔和乳头状瘤消退的动物对病毒抗原的体液免疫反应。用细菌融合蛋白通过Western blotting(免疫印迹)鉴定抗线性表位的抗体，通过免疫沉淀获得识别构象表位的证据。只有一小部分动物对早期蛋白E1、E2、E6和E7有免疫反应，所有动物的E4和E5均为阴性。对E6和E7的反应在7个月左右达到高峰，然后下降，而对E1和E2的反应在最初上升后保持不变。在乳头状瘤阶段，对结构蛋白的抗体反应很低，对L1的抗体主要识别构象表位。随着乳头状瘤进展为癌症，对L1和L2的反应急剧增加，这表明感染病毒的宿主细胞和宿主免疫系统之间的相互作用发生了变化。
Cottontail rabbit papillomavirus induces benign tumors, papillomas, in rabbits which progress at a high frequency to malignant tumors, carcinomas. Cottontail rabbit papillomavirus therefore provides an experimental model for oncogenic human papillomaviruses. The nature of the antigens recognized by the host has not been identified at any stage of tumor development. Here, we characterized the humoral immune response to viral antigens in cottontail and domestic rabbits at the papilloma stage, in domestic rabbits at the carcinoma stage, and in animals in which papillomas had regressed. Antibodies to linear epitopes were identified by Western blotting (immunoblotting) with bacterial fusion proteins, and evidence for recognition of conformational epitopes was obtained by immunoprecipitation. An immune response to the early proteins E1, E2, E6, and E7 was detected only in a fraction of the animals, and all animals were negative for E4 and E5. The response to E6 and E7 peaked around 7 months and then decreased, while that to E1 and E2 remained level after an initial raise. The antibody response to structural proteins was low at the papilloma stage, and antibodies to L1 recognized predominantly conformational epitopes. As papillomas progressed to carcinomas, there was a drastic increase in the response to L1 and L2, suggesting a change in interaction between virus-infected host cells and the host's immune system.