Combined intake of astaxanthin, β-carotene, and resveratrol elevates protein synthesis during muscle hypertrophy in mice
Combined intake of astaxanthin, β-carotene, and resveratrol elevates protein synthesis during muscle hypertrophy in mice
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DOI：
10.1016/j.nut.2019.110561
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发表时间：
2020-01-01
期刊：
影响因子：
4.4
通讯作者：
Higashi, Akane
中科院分区：
文献类型：
--
作者：
Kawamura, Aki;Aoi, Wataru;Higashi, Akane
目的：虾青素、β -胡萝卜素和白藜芦醇等抗氧化因子对骨骼肌中的蛋白质合成有潜在影响，它们的联合摄入可能比单独摄入具有更大的累积效应。本研究旨在探讨萎缩引起的肥厚过程对小鼠骨骼肌质量和蛋白质代谢信号的综合影响。方法：将雄性ICR小鼠分为5组，每组7只：正常、虾青素、β -胡萝卜素、白藜芦醇和所有3种抗氧化剂。各组饲料中分别添加等浓度（0.06% [w/w]）的抗氧化剂。混合组按等比例添加3种抗氧化剂。每只小鼠1条腿拖运3周，诱导肌肉萎缩。取下石膏后，各组饲喂2周。收集肌肉组织，称重，并检查蛋白质代谢信号和氧化损伤。结果：虾青素组、β -胡萝卜素组和白藜芦醇组大鼠结肠肌重量均显著高于正常组；混合抗氧化剂的摄入加速了这一过程（P = 0.007）。混合抗氧化组肌肉中哺乳动物雷帕霉素靶蛋白和p70 S6 K磷酸化水平高于正常组（P = 0.025; P = 0.020）。混合抗氧化剂组羰基化蛋白浓度低于正常组（P = 0.021）。结论：这些结果表明虾青素、β -胡萝卜素和白藜芦醇的组合，即使是少量的，也可以促进肌肉萎缩后肥厚过程中的蛋白质合成。(C) 2019 Elsevier Inc.版权所有。
Objective: The antioxidant factors, astaxanthin, beta-carotene, and resveratrol, have a potential effect on protein synthesis in skeletal muscle and a combined intake may have a greater cumulative effect than individual intake. The aim of this study was to investigate the combined effects on skeletal muscle mass and protein metabolic signaling during the hypertrophic process from atrophy in mice.Methods: Male ICR mice were divided into five dietary groups consisting of seven animals each: normal, astaxanthin, beta-carotene, resveratrol, and all three antioxidants. Equal concentrations (0.06% [w/w]) of the respective antioxidants were included in the diet of each group. In the mixed group, three antioxidants were added in equal proportion. One leg of each mouse was carted for 3 wk to induce muscle atrophy. After removal of the cast, the mice were fed each diet for 2 wk. The muscle tissues were collected, weighed, and examined for protein metabolism signaling and oxidative damage.Results: The weight of the coleus muscle was increased in the astaxanthin, beta-carotene, and resveratrol groups to a greater extent than in the normal group; this was accelerated by intake of the mixed antioxidants (P = 0.007). Phosphorylation levels of mammalian target of rapamycin and p70 S6 K in the muscle were higher in the mixed antioxidant group than in the normal group (P = 0.025; P = 0.020). The carbonylated protein concentration was lower in the mixed antioxidant group than in the normal group (P = 0.021).Conclusions: These results suggested that a combination of astaxanthin, beta-carotene, and resveratrol, even in small amounts, promoted protein synthesis during the muscle hypertrophic process following atrophy. (C) 2019 Elsevier Inc. All rights reserved.