1-Aminocyclopropane-1-carboxylic acid (ACC) deaminase-containing rhizobacteria protect Ocimum sanctum plants during waterlogging stress via reduced ethylene generation
1-Aminocyclopropane-1-carboxylic acid (ACC) deaminase-containing rhizobacteria protect Ocimum sanctum plants during waterlogging stress via reduced ethylene generation
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DOI：
10.1016/j.plaphy.2012.07.008
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发表时间：
2012-09-01
影响因子：
6.5
通讯作者：
Kalra, Alok
中科院分区：
文献类型：
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作者：
Barnawal, Deepti;Bharti, Nidhi;Kalra, Alok
圣罗勒作为雨养作物生长，已知对淹水条件的适应性差。很多时候，作物遭受极端损害，因为缺氧和过量乙烯产生，由于水浸条件下存在大雨。研究了含1-氨基环丙烷-1-羧酸（ACC）脱氨酶的植物根际促生细菌在淹水胁迫下的作用。本研究比较了含ACC脱氨酶的微生物处理对涝渍和非涝渍植物产量和胁迫诱导的生化变化的影响。对10株含ACC-脱氨酶的植物根际促生菌进行了分离和鉴定。筛选出的4个菌株Fd 2（Achromobacter xylosoxidans）、Bac 5（Serratia ureilytica）、Oci 9（Herbaspirillum seropedicae）和Oci 13（Ochrobactrum roizsphaelae）具有保护罗勒植物免受水淹的潜力。通过盆栽试验，评价了这些ACC脱氨酶筛选菌株在降低涝渍条件下产量损失方面的潜力。细菌处理保护植物免受水涝诱导的有害变化，如应力乙烯生产，叶绿素浓度降低，更高的脂质过氧化，脯氨酸浓度和减少叶片养分吸收。Fd2（A.在正常的非淹水条件下，接种细菌后的植株表现出最大的耐涝性，在胁迫乙烯水平最低的条件下，处理植株的生长和草本产量最高（比未接种细菌的植株高46.5%）（ACC浓度比未接种细菌的植株低53%），而在正常的非淹水条件下，O.根际土壤对植物生长的促进作用最强。(C)2012年Elsevier Masson SAS。All rights reserved.
Ocimum sanctum grown as rain-fed crop, is known to be poorly adapted to waterlogged conditions. Many a times the crop suffers extreme damages because of anoxia and excessive ethylene generation due to waterlogging conditions present under heavy rain. The usefulness of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-containing plant growth promoting rhizobacteria was investigated under waterlogging stress. The comparison of herb yield and stress induced biochemical changes of waterlogged and non-waterlogged plants with and without ACC deaminase-containing microbiological treatments were monitored in this study. Ten plant growth promoting rhizobacteria strains containing ACC-deaminase were isolated and characterized. Four selected isolates Fd2 (Achromobacter xylosoxidans), Bac5 (Serratia ureilytica), Oci9 (Herbaspirillum seropedicae) and Oci13 (Ochrobactrum rhizosphaerae) had the potential to protect Ocimum plants from flood induced damage under waterlogged glass house conditions. Pot experiments were conducted to evaluate the potential of these ACC deaminase-containing selected strains for reducing the yield losses caused by waterlogging conditions. Bacterial treatments protected plants from waterlogging induced detrimental changes like stress ethylene production, reduced chlorophyll concentration, higher lipid peroxidation, proline concentration and reduced foliar nutrient uptake. Fd2 (A. xylosoxidans) induced maximum waterlogging tolerance as treated waterlogged plants recorded maximum growth and herb yield (46.5% higher than uninoculated waterlogged plants) with minimum stress ethylene levels (53% lower ACC concentration as compared to waterlogged plants without bacterial inoculation) whereas under normal non-waterlogged conditions O. rhizosphaerae was most effective in plant growth promotion. (C) 2012 Elsevier Masson SAS. All rights reserved.