AKT signalling and mitochondrial pathways are involved in mushroom polysaccharide-induced apoptosis and G1 or S phase arrest in human hepatoma cells
AKT signalling and mitochondrial pathways are involved in mushroom polysaccharide-induced apoptosis and G1 or S phase arrest in human hepatoma cells
复制标题
AKT 信号和线粒体途径参与蘑菇多糖诱导的人肝癌细胞凋亡和 G1 或 S 期停滞
DOI：
10.1016/j.foodchem.2012.10.047
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发表时间：
2013-06-15
期刊：
影响因子：
8.8
通讯作者：
Zhao, Min
中科院分区：
文献类型：
--
作者：
OuYang, Fengju;Wang, Guibin;Zhao, Min
This study describes molecular mechanisms for inhibiting tumour cell proliferation using polysaccharides from medicinal mushrooms in human hepatoma cells. The results show that regarding cell cycle-related proteins, three types of polysaccharides significantly enhance the expression of p27(Kip) in HepG2 and Bel-7404 cells, while suppressing the activity of cyclin D1/CDK4 and/or cyclin E/CDK2. Considering apoptosis-related factors, the polysaccharides suppressed ART activity through the inhibition of ART phosphorylation at Thr(308) and/or Ser(473). The growth of HepG2 and Bel-7404 cells was suppressed by the up-regulation of a subunit of PI3K and phospho-PTEN, which are modulators of AKT activity. The polysaccharides also activated the mitochondria-mediated apoptosis pathway by stimulating the activation of BcI-2 family proteins to release cytochrome c and Smac and cleave caspase-9 and caspase-3 in HepG2 and Bel-7404 cells. These factors have a potent effect on cell cycle arrest in G(1) and/or S phase and induce apoptosis in HepG2 and Bel-7404 cells. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.
