Rapid discrimination by flow cytometry between viable and non‐viable Vero cells treated with Clostridium perfringens enterotoxin
Rapid discrimination by flow cytometry between viable and non‐viable Vero cells treated with Clostridium perfringens enterotoxin
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通过流式细胞术快速区分经产气荚膜梭菌肠毒素处理的活细胞和非活细胞 Vero 细胞
DOI：
10.1111/j.1472-765x.1988.tb01236.x
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发表时间：
1988
影响因子：
2.4
通讯作者：
Y. Ushijima
中科院分区：
文献类型：
--
作者：
T. Uemura;G. Sakaguchi;T. Yamashita;Y. Ushijima
用产气荚膜梭菌肠毒素（CPE）处理后，然后用荧光素二乙酸酯（FDA）和碘化丙啶（PI）同时双重染色，用流式细胞仪（FCM）对活的和非活的非洲绿色猴肾（Vero）细胞进行计数。在1分钟内，FCM分析了样品中10000个Vero细胞的活力。用CPE处理Vero细胞60 min并用FDA-PI染色5 min后，在25 - 400 ng/ml CPE和活细胞数百分比之间获得了可重现的剂量反应曲线。FCM分析被证明是一种强有力的工具，可快速区分大量样本中经CPE处理的活细胞和非活细胞。
Viable and non‐viable African green monkey kidney (Vero) cells after treatment with Clostridium perfringens enterotoxin (CPE) followed by simultaneous double staining with fluorescein diacetate (FDA) and propidium iodide (PI) were counted with a flow cytometer (FCM). Within 1 min the FCM analysed 10 000 Vero cells in a sample for viability. After treatment of Vero cells with CPE for 60 min and staining with FDA‐PI for 5 min, a reproducible dose‐response curve was obtained between 25 and 400 ng/ml of CPE and percentage viable cell numbers. The FCM analysis proved to be a strong tool for rapid discrimination between viable and non‐viable Vero cells treated with CPE in a large number of samples at a time.