Ontogeny of the thymus in a teleost fish, Cyprinus carpio L.:: developing thymocytes in the epithelial microenvironment
Ontogeny of the thymus in a teleost fish, Cyprinus carpio L.:: developing thymocytes in the epithelial microenvironment
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DOI：
10.1016/s0145-305x(98)00053-6
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发表时间：
1999-03-01
影响因子：
2.9
通讯作者：
Rombout, JHMW
中科院分区：
文献类型：
--
作者：
Romano, N;Taverne-Thiele, AJ;Rombout, JHMW
一种单克隆抗体，WCL 9，特异性的胸腺细胞亚群的膜分子被用来检测这些细胞在胸腺的个体发育过程中原位。冷冻切片显示胸腺（受精后第4天）的雏形中存在WCL 9（+）细胞;此后，从胸腺区域化首次出现（受精后第3周）直至成年，仅在皮质中观察到阳性细胞。用WCL 9对胸腺进行整体免疫染色，通过特异性染色揭示了皮质的三维结构。用原位末端标记法研究了胸腺发育过程中凋亡细胞的存在和分布。从受精后第4周起，凋亡细胞更频繁地检测到皮质比髓质，表明在皮质胸腺细胞的连续选择。超微结构研究证实巨噬细胞内存在大量皮质凋亡细胞，电子显微镜为胸腺上皮异质性的存在提供了证据。在个体发育过程中，上皮细胞的分化，从第一周，直到少年年龄。细胞类型根据其定位和细胞学特征分类为：i）位于被膜下的限制性上皮细胞。血管周围和小梁周围区; ii）位于髓质和皮质区的网状上皮细胞; iii）皮质和髓质之间边界处的护士样细胞; iii）位于髓质中的Hassall小体样结构。这项研究表明，在胸腺细胞分化的过程中，发生了广泛的淋巴上皮相互作用。(C)1999爱思唯尔科技有限公司。保留所有权利。
A monoclonal antibody, WCL9, specific for membrane molecules of a thymocyte subpopulation was used to detect these cells in situ during the ontogeny of thymus. Cryo-sections revealed WCL9(+) cells in the rudiment of the thymus (day 4 post fertilization); thereafter, the positive cells were observed exclusively in the cortex from the first appearance of thymic regionalization (week 3 post fertilization) until adult age. Whole-mount immunostaining of the thymus with WCL9 revealed the three-dimensional structure of the cortex by specific staining. The presence and distribution of apoptotic cells during thymus development was studied by in situ end-labelling of fragmented DNA. From week 4 post fertilization onwards, apoptotic cells were more frequently detected in the cortex than medulla, suggesting a continuous selection of thymocytes in the cortex. Ultrastructural studies confirmed the presence of numerous cortical apoptotic cells inside macrophages.Electron microscopy provided evidence for the existence of epithelial heterogeneity in the thymus. During the ontogeny, the differentiation of epithelial cells was followed from the first weeks until the juvenile age. Cell types were classified on the basis of their localization and cytological characteristics as: i) limiting epithelial cells located in subcapsular. perivascular and peritrabecular zones; ii) reticular epithelial cells situated in medullary and cortical zones; iii) nurse-like cells at the border between the cortex and medulla, iiii) Hassall's body-like structures localized in the medulla. This study could suggest the occurrence of a wide range of lympho-epithelial interactions throughout thymocytes differentiation. (C) 1999 Elsevier Science Ltd. All rights reserved.