PURIFICATION OF ACTIVATED HAGEMAN FACTOR (ACTIVATED FACTOR XII)
PURIFICATION OF ACTIVATED HAGEMAN FACTOR (ACTIVATED FACTOR XII)
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DOI：
10.1021/bi00912a005
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发表时间：
1962-01-01
期刊：
影响因子：
2.9
通讯作者：
DAVIE, EW
中科院分区：
文献类型：
--
作者：
RATNOFF, OD;DAVIE, EW
本文报道了用羧甲基纤维素和二乙氨基乙基纤维素柱层析法从正常人血浆中纯化活化的Hageman因子的方法。产物纯化3000- 5000倍或更多，如通过新的测定程序测量的。纯化制剂纠正了Hageman性状患者血浆中的凝血缺陷，加速了硅胶管中正常血浆的凝血，并将血浆促凝血酶原激酶前体转化为活化形式。该制剂可能被微量纤溶酶原污染，也可能被圣诞因子污染。高度纯化的活化Hageman因子不被10-3 M二异丙基磷酰氟抑制。
A method is describedfor the purification of activated Hageman factor from normal human plasma, utilizing column chromatography with carboxy-methylcellulose and diethylaminoethyl cellulose. The product was purified 3000- to 5000-fold or more as measured by a new assay procedure. The purified preparations corrected the clotting defect in the plasma of patients with Hageman trait, accelerated the clotting of normal plasma in silicone tubes, and converted plasma thromboplastin antecedent to an activated form. The preparation was probably contaminated with traces of plasminogen and perhaps Christmas factor as well. The highly purified activated Hageman factor was not inhibited by 10-3 M diisopropyl phosphofluoridate.