Selenoprotein mRNA is expressed in blood at levels comparable to major tissues in rats
Selenoprotein mRNA is expressed in blood at levels comparable to major tissues in rats
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DOI：
10.1093/jn/134.10.2640
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发表时间：
2004-10-01
影响因子：
4.2
通讯作者：
Sunde, RA
中科院分区：
文献类型：
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作者：
Evenson, JK;Wheeler, AD;Sunde, RA
相对于其他参数，肝脏谷胱甘肽过氧化物酶-1(GPX1)基因的表达受硒状态的高度调控，但在测定人体硒需要量方面的作用有限。为了验证使用分子生物学标记物进行血液硒状态评估的有效性，我们使用核糖核酸酶保护试验(RPA)研究了全血中相对于其他16个大鼠组织的mRNA水平。从1毫升全血中提取到显著数量的总RNA(>；50微克)。对断奶后28天缺硒(0.2杯/g饲料)雄性大鼠的总RNA进行了GPX1、GpX4、GPX3、硫氧还蛋白还原酶-1(TRR1)和硒蛋白-P(SELP)的分析。RPA检测到至少1种硒蛋白在除胰腺外的所有组织中都有显著的mRNA表达。使用这种混合的RPA探针表达的GPX1在除睾丸以外的所有组织中产生的信号相对于其他硒蛋白信号最高；GPX1在血液中的表达排名第四，与主要器官(肝脏，第一；心脏，第五；肾脏，第六)的表达相似。肾脏中GPX3的含量最高，睾丸中Gpx4、TRR1和SELP的含量最高。这项研究首次报道了一些硒蛋白的基因表达模式，并跨越了一系列全面的组织。血液中所有硒蛋白的mRNA水平与主要器官中的水平相当，缺硒时血液和肝脏中GPX1mRNA水平的下降趋势相似，支持使用全血来评估分子生物学标记的硒状况。
Liver glutathione peroxidase-1 (GPX1) mRNA is highly regulated by Se status relative to other parameters, but is of limited use for determining Se requirements in humans. To examine the efficacy of using blood for Se status assessment using molecular biology markers, we used a ribonuclease protection assay (RPA) to study mRNA levels in whole blood relative to 16 other rat tissues. Significant amounts of total RNA (>50 mug) were obtained from 1 mL of whole blood. Total RNA from 28-d postweaning Se-adequate (0.2 mug Se/g diet) male rats was analyzed for GPX1, GPX4, GPX3, thioredoxin reductase-1 (TRR1), and selenoprotein-P (SelP). RPA detected significant mRNA expression for at least 1 selenoprotein in all tissues except pancreas. GPX1 mRNA expression using this mix of RPA probes yielded the highest signal for GPX1 relative to the other selenoprotein signals in all tissues except testis; GPX1 expression was 4th highest in blood and similar to the major organs (liver, 1st; heart, 5th; kidney, 6th). Kidney was highest for GPX3, and testes was highest for GPX4, TRR1, and SelP. This study is the first to report the gene expression pattern for a number of selenoproteins and across a comprehensive set of tissues. The mRNA levels for all selenoproteins in blood were comparable to levels in the major organs, and decreases in blood and liver GPX1 mRNA levels in Se deficiency were similar, supporting potential use of whole blood for assessing Se status using molecular biology markers.