Imaging and Isolation of Extravasation-Participating Endothelial and Melanoma Cells During Angiopellosis.
Imaging and Isolation of Extravasation-Participating Endothelial and Melanoma Cells During Angiopellosis.
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DOI：
10.1007/978-1-0716-1205-7_30
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Cheng K
中科院分区：
文献类型：
--
作者：
Allen TA;Cheng K
癌症死亡率主要是癌症转移的结果。最近显微镜技术的进步使得循环肿瘤细胞（ctc）在血管外（出）的成像成为可能，这是转移过程中的关键步骤。在这里，我们描述了使用活体显微镜技术来成像和分离外渗性黑色素瘤ctc和参与外渗的内皮细胞。这些技术可以作为研究肿瘤转移的手段和抗癌治疗的筛选工具。
Cancer mortality rates are primarily a result of cancer metastasis. Recent advances in microscopy technology allow for the imaging of circulating tumor cells (CTCs) as they extravasate (exit) blood vessels, a key step in the metastasis process. Here, we describe the use of intravital microscopy techniques to image and isolate both extravasating melanoma CTCs and the extravasation-participating endothelial cells. These techniques can be used as a means to study cancer metastasis and as a screening tool for anticancer therapeutics.