Mitogenic activity of sulfated chitosan and cellulose derivatives is related to protection of FGF-2 from proteolytic cleavage.
Mitogenic activity of sulfated chitosan and cellulose derivatives is related to protection of FGF-2 from proteolytic cleavage.
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DOI：
10.1002/mabi.201100518
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发表时间：
2012-06
影响因子：
4.6
通讯作者：
Annett Weltrowski;Marie-Luise da Silva Almeida;D. Peschel;Kai Zhang;S. Fischer;T. Groth
中科院分区：
文献类型：
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作者：
Annett Weltrowski;Marie-Luise da Silva Almeida;D. Peschel;Kai Zhang;S. Fischer;T. Groth
新型壳聚糖（CHS）和纤维素硫酸盐（CS）的研究有关其促有丝分裂活性和其对FGF-2的蛋白水解消化的保护作用。中等硫酸化程度（DS（S））和较低浓度的CHS对3 T3细胞生长具有上级作用，而CS的促有丝分裂活性随着DS（S）和浓度的增加而增加。以胰蛋白酶为模型蛋白酶的实验表明，保护FGF-2免受蛋白水解消化取决于DS（S）和衍生物的浓度，其方式与细胞生长相同。对添加到3 T3细胞培养物中的FGF-2的稳定性的研究表明，在衍生物的存在下，FGF-2浓度保持较高。结果表明，CHS和CS的促有丝分裂活性是由于保护FGF-2免受蛋白水解切割。
Novel chitosan (CHS) and cellulose sulfates (CSs) are studied regarding their mitogenic activity and their protective effect against proteolytic digestion of FGF-2. An intermediate degree of sulfation (DS(S) ) and lower concentration of CHS have superior effect on 3T3 cell growth while the mitogenic activity of CS increases with DS(S) and concentration. Experiments with trypsin as model proteinase show that protection of FGF-2 from proteolytic digestion depends on DS(S) and the concentration of derivatives in the same manner as cell growth. Studies on stability of FGF-2 added to cultures of 3T3 cells show that the FGF-2 concentration remains higher in the presence of derivatives. Results indicate that the mitogenic activity of CHS and CS is due to protection of FGF-2 from proteolytic cleavage.