Tissue Culture Methods
Tissue Culture Methods
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组织培养方法
DOI：
10.1007/978-1-4612-6076-9_7
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发表时间：
1980
影响因子：
1.3
通讯作者：
H. Oberlander
中科院分区：
文献类型：
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作者：
H. Oberlander
1915年，当时在耶鲁大学的理查德·戈德施泰特发表了一篇简短的文章，描述了第一次成功的昆虫组织器官培养。他关于Cecropia精母细胞的论文以这样的话开头：“出乎意料的情况迫使我中断了正常的工作，这为我提供了一个尝试一些实验的机会，我已经考虑了一段时间，将组织培养的方法应用于性细胞的研究，目的是使这些细胞的历史可以用于实验分析”（Goldschlauf 1915 p.220;斜体H.O.）。他对组织培养在昆虫生理学研究中的实用性的认识是有预见性的。然而，直到过去十年，组织培养技术才被昆虫生理学家广泛使用。体外方法在昆虫生理学和发育问题上的应用取得了巨大成功（Kurstak和Maramorosch 1976; Maramorosch 1976）。现在，在培养皿中用假定的组织在营养培养基中与适当的激素一起孵育产生翅膀或腿已经是一件例行公事了。在这样的实验中，昆虫在成为感兴趣的组织的供体之后被丢弃。在已经从昆虫组织建立连续细胞系的情况下，根本不需要维持昆虫。
In 1915 Richard Goldschmidt, then at Yale, published a brief note that described the first successful organ culture of an insect tissue. His paper on Cecropia spermatocytes began with the words, “Unforseen circumstances, that forced me to interrupt my normal work offered a chance for trying some experiments that I have had in mind for some time, to apply the method of tissue culture to the study of sex cells with the purpose of rendering the history of these cells accessible to experimental analysis” (Goldschmidt 1915 p. 220; italics H.O.). His recognition of the utility of tissue culture for the study of insect physiology was prophetic. However, it was not until the past decade that tissue culture techniques became widely used by insect physiologists. The application of in vitro methods to problems in insect physiology and development has met with outstanding success (Kurstak and Maramorosch 1976; Maramorosch 1976). It is now a matter of routine to produce wings or legs in a petri dish from presumptive tissues incubated with the appropriate hormones in a nutritive medium. In such experiments, the insect is discarded after it has been the donor of the tissue of interest. In instances where continuous cell lines have been established from insect tissues, insects need not be maintained at all.