Hydrophilic magnetic molecularly imprinted nanobeads for efficient enrichment and high performance liquid chromatographic detection of 17beta-estradiol in environmental water samples.
Hydrophilic magnetic molecularly imprinted nanobeads for efficient enrichment and high performance liquid chromatographic detection of 17beta-estradiol in environmental water samples.
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DOI：
10.1016/j.talanta.2020.121367
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发表时间：
2020-07
期刊：
影响因子：
6.1
通讯作者：
Xing Tian;Huijia Song;Yue Wang;Xuemeng Tian;Yuhai Tang;Ruixia Gao;Chengxiao Zhang
中科院分区：
文献类型：
--
作者：
Xing Tian;Huijia Song;Yue Wang;Xuemeng Tian;Yuhai Tang;Ruixia Gao;Chengxiao Zhang
采用分子印迹聚合物在水性溶剂中在磁性纳米珠表面合成了新型17β-雌二醇（E2）磁性分子印迹纳米珠，即E2-MMINs。以羧基功能化的Fe3O4纳米粒子为载体，E2为模板分子，多巴胺为功能单体构建了亲水性纳米珠。对合成的E2-MMINs进行了不同方面的研究，包括合成条件、理化性质和吸附条件。实验结果表明，E2-MMINs不仅具有薄的印迹层、稳定的晶型和快速的磁分离能力，而且具有快速的动力学（20 min）、高的结合量（41.48 mg g−1）、良好的特异性（印迹因子= 8.07）和良好的重复使用性（重复使用10次后吸附效率> 94.8%）。此外，还开发了E2-MMINs结合高效液相色谱对痕量E2进行特异性富集和测定的低LOD（0.008μgL−1）方法，并在环境水样中得到了应用。这项工作表明，所提出的合成策略对于合成亲水性纳米珠以有效富集和检测目标分子 E2 非常有前景。
Novel magnetic molecularly imprinted nanobeads for 17β-estradiol (E2), namely, E2-MMINs, were synthesized by molecularly imprinted polymers on the surface of magnetic nanobeads in aqueous solvents. The hydrophilic nanobeads were set up by adopting carboxyl group-functionalized Fe3O4nanoparticles as carriers, E2 as template molecule, and dopamine as functional monomer. The synthesized E2-MMINs were investigated in different aspects including synthesis conditions, physical and chemical properties, and adsorption conditions. The experimental results show that the E2-MMINs present not only thin imprinting layers, stable crystal form, and fast magnetic separation ability, but also rapid kinetics (20 min), high binding amount (41.48 mg g−1), satisfactory specificity (imprinting factor = 8.07), and favorable reusability (adsorption efficiency > 94.8% after reusing for 10 times). Moreover, the method employing E2-MMINs combined with high performance liquid chromatography for the specific enrichment and determination of trace E2 has been developed with the low LOD (0.008 μg L−1), and the application in environmental water samples has been proved. This work demonstrates that the proposed synthetic strategy is highly promising to the synthesis of hydrophilic nanobeads for efficient enrichment and detection of target molecule E2.