Genome-wide identification and expression profiling of the SOX gene family in a bivalve mollusc Patinopecten yessoensis
Genome-wide identification and expression profiling of the SOX gene family in a bivalve mollusc Patinopecten yessoensis
复制标题
双壳类软体动物虾夷扇贝 SOX 基因家族的全基因组鉴定和表达谱
DOI：
10.1016/j.gene.2017.07.013
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发表时间：
2017
期刊：
影响因子：
3.5
通讯作者：
Bao Zhenmin
中科院分区：
文献类型：
--
作者：
Yu Jiachen;Zhang Lingling;Li Yangping;Li Ruojiao;Zhang Meiwei;Li Wanru;Xie Xinran;Wang Shi;Hu Xiaoli;Bao Zhenmin
SOX家族是一个转录因子家族，在多种发育过程中发挥重要作用。全面了解SOX家族的进化需要对SOX基因在不同门中的特性进行全面的研究。软体动物是后生动物中的第二大门，但迄今为止，对SOX家族的系统研究还很缺乏。在这项研究中，我们进行了全基因组鉴定的SOX家族在虾夷扇贝和描绘他们的组织分布和时间表达模式在卵巢和睾丸配子发生。鉴定出7个SOX基因，包括SOXB 1、B2、C、D、E、F和H，代表了原口类中第一个SOX成员与脊索动物最后共同祖先中存在的SOX成员相同的记录。基因组结构分析确定了相对保守的外显子-内含子结构，伴随着内含子插入。实时荧光定量PCR分析显示SOX可能参与了扇贝的多种功能，包括神经感觉细胞分化、造血、肌肉发生和配子发生。本研究首次对软体动物SOX基因家族进行了系统的研究。这将有助于更好地了解SOX家族在后生动物中的进化和功能。
SOX family is composed of transcription factors that play vital roles in various developmental processes. Comprehensive understanding on evolution of the SOX family requires full characterization of SOX genes in different phyla. Mollusca is the second largest metazoan phylum, but till now, systematic investigation on the SOX family is still lacking in this phylum. In this study, we conducted genome-wide identification of the SOX family in Yesso scallop Patinopecten yessoensis and profiled their tissue distribution and temporal expression patterns in the ovaries and testes during gametogenesis. Seven SOX genes were identified, including SOXB1, B2, C, D, E, F and H, representing the first record in protostomes with SOX members identical to that proposed to exist in the last common ancestor of chordates. Genomic structure analysis identified relatively conserved exon-intron structures, accompanied by intron insertion. Quantitative real-time PCR analysis revealed possible involvement of scallop SOX in various functions, including neuro-sensory cell differentiation, hematopoiesis, myogenesis and gametogenesis. This study represents the first systematic characterization of SOX gene family in Mollusca. It will assist in a better understanding of the evolution and function of SOX family in metazoans.