Ganoderma lucidum polysaccharide modulates gut microbiota and immune cell function to inhibit inflammation and tumorigenesis in colon
Ganoderma lucidum polysaccharide modulates gut microbiota and immune cell function to inhibit inflammation and tumorigenesis in colon
复制标题
灵芝多糖可调节肠道微生物群和免疫细胞功能，从而抑制结肠炎症与肿瘤发生。
DOI：
10.1016/j.carbpol.2021.118231
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发表时间：
2021-05-26
影响因子：
11.2
通讯作者：
Wang, Xingya
中科院分区：
文献类型：
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作者：
Guo, Cuiling;Guo, Dandan;Wang, Xingya
本研究探讨了从灵芝去孢子皮孢子中提取的水溶性多糖（GLP）对 AOM/DSS 诱导的炎症、肿瘤发生和肠道菌群改变的影响，这在之前从未有过报道。我们的数据显示，GLP（200 和 300 mg/kg）可减少 AOM/DSS 诱导的结肠炎和肿瘤发生，表现为疾病活动指数评分以及肿瘤总数和大小显着降低。此外，GLP 还可以通过抑制 TLR4/MyD88/NF-kappa B 信号传导改善 AOM/DSS 诱导的微生物群失调、增加短链脂肪酸的产生并减轻内毒素血症。此外，GLP 显着改善了肠道屏障功能，杯状细胞数量增加、MUC2 分泌和紧密连接蛋白表达增加证明了这一点。 GLP 治疗抑制巨噬细胞浸润并下调 IL-1β、iNOS 和 COX-2 表达。此外，GLP 还可抑制巨噬细胞 RAW264.7、肠道 HT-29 和 NCM460 细胞中脂多糖 (LPS) 诱导的炎症标志物和 MAPK（JNK 和 ERK）激活。总之，这些结果表明 GLP 是一种有前途的治疗结直肠癌的益生元。
This study investigated the effects of water-soluble polysaccharide extracted from the sporoderm-removed spores of Ganoderma lucidum (GLP) against AOM/DSS-induced inflammation, tumorigenesis, and gut microbiota modification, which has never been reported before. Our data revealed that GLP (200 and 300 mg/kg) decreased AOM/DSS-induced colitis and tumorigenesis, manifested by significantly reduced disease activity index score, and total number and size of tumors. Furthermore, GLP ameliorated AOM/DSS-induced microbiota dysbiosis, increased short-chain fatty acid production, and alleviated endotoxemia by inhibiting TLR4/MyD88/NF-kappa B signaling. Besides, GLP profoundly improved gut barrier function as evidenced by increased numbers of goblet cells, MUC2 secretion, and tight junction protein expressions. GLP treatment inhibited macrophage infiltration and downregulated IL-1 beta, iNOS, and COX-2 expressions. Additionally, GLP inhibited lipopolysaccharides (LPS)induced inflammation markers and MAPK (JNK and ERK) activation in macrophage RAW264.7, intestinal HT-29, and NCM460 cells. In conclusion, these results indicate that GLP is a promising prebiotic for the treatment of colorectal cancer.